CD276, CPTAC-6221 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CD276	B7H3

Sequence Length (AA)	Molecular Weight (Da)
534	57235

Protein Name
CD276 antigen

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MLRRRGSPGM	GVHVGAALGA	LWFCLTGALE	VQVPEDPVVA	LVGTDATLCC
60	70	80	90	100
SFSPEPGFSL	AQLNLIWQLT	DTKQLVHSFA	EGQDQGSAYA	NRTALFPDLL
110	120	130	140	150
AQGNASLRLQ	RVRVADEGSF	TCFVSIRDFG	SAAVSLQVAA	PYSKPSMTLE
160	170	180	190	200
PNKDLRPGDT	VTITCSSYQG	YPEAEVFWQD	GQGVPLTGNV	TTSQMANEQG
210	220	230	240	250
LFDVHSILRV	VLGANGTYSC	LVRNPVLQQD	AHSSVTITPQ	RSPTGAVEVQ
260	270	280	290	300
VPEDPVVALV	GTDATLRCSF	SPEPGFSLAQ	LNLIWQLTDT	KQLVHSFTEG
310	320	330	340	350
RDQGSAYANR	TALFPDLLAQ	GNASLRLQRV	RVADEGSFTC	FVSIRDFGSA
360	370	380	390	400
AVSLQVAAPY	SKPSMTLEPN	KDLRPGDTVT	ITCSSYRGYP	EAEVFWQDGQ
410	420	430	440	450
GVPLTGNVTT	SQMANEQGLF	DVHSVLRVVL	GANGTYSCLV	RNPVLQQDAH
460	470	480	490	500
GSVTITGQPM	TFPPEALWVT	VGLSVCLIAL	LVALAFVCWR	KIKQSCEEEN
510	520	530	534
AGAEDQDGEG	EGSKTALQPL	KHSDSKEDDG	QEIA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-6221 Collapse assay details

Data source: Panorama

Official Gene Symbol: CD276
Peptide Modified Sequence: DLRPGDTVTITC[+57.021464]SSYR
Modification Type: Carbamidomethyl Cysteine
Protein - Site of Modification: N/A
Peptide - Site of Modification: 7
Peptide Start: 372
Peptide End: 387

CPTAC ID: CPTAC-6221
Peptide Molecular Mass: 1,839.8785
Species: Homo Sapiens
Assay Type: Enrichment MRM
Enrichment Method: Peptide Immunoaffinity
Matrix: Frozen tissue
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTRAP 5500
Internal Standard: Extended heavy peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Eksigent
Column Packing: Reprosil C18, 3um
Column Dimensions: 75µm x 15cm
Flow Rate: 0.3 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	5	3.3	1.2	7.8	4.3	4.2	9.3	5.4	4.4	15	15	15
y6 (1+)	5.2	3.1	1.7	8.3	4.2	4.3	9.8	5.2	4.6	15	15	15
b7 (1+)	4	3.2	1.6	9.5	4.3	3.8	10.3	5.4	4.1	15	15	15
b8 (1+)	5.4	3.5	1.8	8.5	4.1	4.9	10.1	5.4	5.2	15	15	15
sum	4.9	3.2	1.3	8.3	4.2	4.2	9.6	5.3	4.4	15	15	15

CD276

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-6221 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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CD276

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-6221 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments