Show Nav →
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
PAXIP1 PAXIP1L, PTIP
Sequence Length (AA) Molecular Weight (Da)
1069 121341
Protein Name
PAX-interacting protein 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSDQAPKVPE EMFREVKYYA VGDIDPQVIQ LLKAGKAKEV SYNALASHII
60 70 80 90 100
SEDGDNPEVG EAREVFDLPV VKPSWVILSV QCGTLLPVNG FSPESCQIFF
110 120 130 140 150
GITACLSQVS SEDRSALWAL VTFYGGDCQL TLNKKCTHLI VPEPKGEKYE
160 170 180 190 200
CALKRASIKI VTPDWVLDCV SEKTKKDEAF YHPRLIIYEE EEEEEEEEEE
210 220 230 240 250
VENEEQDSQN EGSTDEKSSP ASSQEGSPSG DQQFSPKSNT EKSKGELMFD
260 270 280 290 300
DSSDSSPEKQ ERNLNWTPAE VPQLAAAKRR LPQGKEPGLI NLCANVPPVP
310 320 330 340 350
GNILPPEVRG NLMAAGQNLQ SSERSEMIAT WSPAVRTLRN ITNNADIQQM
360 370 380 390 400
NRPSNVAHIL QTLSAPTKNL EQQVNHSQQG HTNANAVLFS QVKVTPETHM
410 420 430 440 450
LQQQQQAQQQ QQQHPVLHLQ PQQIMQLQQQ QQQQISQQPY PQQPPHPFSQ
460 470 480 490 500
QQQQQQQAHP HQFSQQQLQF PQQQLHPPQQ LHRPQQQLQP FQQQHALQQQ
510 520 530 540 550
FHQLQQHQLQ QQQLAQLQQQ HSLLQQQQQQ QIQQQQLQRM HQQQQQQQMQ
560 570 580 590 600
SQTAPHLSQT SQALQHQVPP QQPPQQQQQQ QPPPSPQQHQ LFGHDPAVEI
610 620 630 640 650
PEEGFLLGCV FAIADYPEQM SDKQLLATWK RIIQAHGGTV DPTFTSRCTH
660 670 680 690 700
LLCESQVSSA YAQAIRERKR CVTAHWLNTV LKKKKMVPPH RALHFPVAFP
710 720 730 740 750
PGGKPCSQHI ISVTGFVDSD RDDLKLMAYL AGAKYTGYLC RSNTVLICKE
760 770 780 790 800
PTGLKYEKAK EWRIPCVNAQ WLGDILLGNF EALRQIQYSR YTAFSLQDPF
810 820 830 840 850
APTQHLVLNL LDAWRVPLKV SAELLMSIRL PPKLKQNEVA NVQPSSKRAR
860 870 880 890 900
IEDVPPPTKK LTPELTPFVL FTGFEPVQVQ QYIKKLYILG GEVAESAQKC
910 920 930 940 950
THLIASKVTR TVKFLTAISV VKHIVTPEWL EECFRCQKFI DEQNYILRDA
960 970 980 990 1000
EAEVLFSFSL EESLKRAHVS PLFKAKYFYI TPGICPSLST MKAIVECAGG
1010 1020 1030 1040 1050
KVLSKQPSFR KLMEHKQNSS LSEIILISCE NDLHLCREYF ARGIDVHNAE
1060 1069
FVLTGVLTQT LDYESYKFN

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for CPTAC-6224 Collapse assay details

Data source: Panorama

Official Gene Symbol
PAXIP1
Peptide Sequence
IIQAHGGTVDPTFTSR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
7
Peptide Start
632
Peptide End
647
CPTAC ID
CPTAC-6224
Peptide Molecular Mass
1,698.8689
Species
Homo Sapiens
Assay Type
Enrichment MRM
Enrichment Method
Peptide Immunoaffinity
Matrix
Frozen tissue
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTRAP 5500
Internal Standard
Extended heavy peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Eksigent
Column Packing
Reprosil C18, 3um
Column Dimensions
75µm x 15cm
Flow Rate
0.3 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 7.2 2.5 3.4 7.2 3.1 5.3 10.2 4 6.3 15 15 15
b10 (1+) 8 3.1 4 7.5 3.7 5.9 11 4.8 7.1 15 15 15
y14 (2+) 8.5 3.8 4.3 8.2 3.8 6 11.8 5.4 7.4 15 15 15
y6 (2+) 7.3 3.4 3 6.3 3.5 5.8 9.6 4.9 6.5 15 15 15
sum 7.5 2.9 3.2 6.7 3.3 5.4 10.1 4.4 6.3 15 15 15


Additional Resources and Comments