MUTYH, CPTAC-6229 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
MUTYH	MYH

Sequence Length (AA)	Molecular Weight (Da)
546	60069

Protein Name
Adenine DNA glycosylase

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MTPLVSRLSR	LWAIMRKPRA	AVGSGHRKQA	ASQEGRQKHA	KNNSQAKPSA
60	70	80	90	100
CDGMIAECPG	APAGLARQPE	EVVLQASVSS	YHLFRDVAEV	TAFRGSLLSW
110	120	130	140	150
YDQEKRDLPW	RRRAEDEMDL	DRRAYAVWVS	EVMLQQTQVA	TVINYYTGWM
160	170	180	190	200
QKWPTLQDLA	SASLEEVNQL	WAGLGYYSRG	RRLQEGARKV	VEELGGHMPR
210	220	230	240	250
TAETLQQLLP	GVGRYTAGAI	ASIAFGQATG	VVDGNVARVL	CRVRAIGADP
260	270	280	290	300
SSTLVSQQLW	GLAQQLVDPA	RPGDFNQAAM	ELGATVCTPQ	RPLCSQCPVE
310	320	330	340	350
SLCRARQRVE	QEQLLASGSL	SGSPDVEECA	PNTGQCHLCL	PPSEPWDQTL
360	370	380	390	400
GVVNFPRKAS	RKPPREESSA	TCVLEQPGAL	GAQILLVQRP	NSGLLAGLWE
410	420	430	440	450
FPSVTWEPSE	QLQRKALLQE	LQRWAGPLPA	THLRHLGEVV	HTFSHIKLTY
460	470	480	490	500
QVYGLALEGQ	TPVTTVPPGA	RWLTQEEFHT	AAVSTAMKKV	FRVYQGQQPG
510	520	530	540	546
TCMGSKRSQV	SSPCSRKKPR	MGQQVLDNFF	RSHISTDAHS	LNSAAQ

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

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Assay Details for CPTAC-6218 Collapse assay details

Data source: Panorama

Official Gene Symbol: MUTYH
Peptide Modified Sequence: M[+15.994915]GQQVLDNFFR
Modification Type: Oxidation (M)
Protein - Site of Modification: N/A
Peptide - Site of Modification: 7
Peptide Start: 521
Peptide End: 531

CPTAC ID: CPTAC-6218
Peptide Molecular Mass: 1,369.6449
Species: Homo Sapiens
Assay Type: Enrichment MRM
Enrichment Method: Peptide Immunoaffinity
Matrix: Frozen tissue
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTRAP 5500
Internal Standard: Extended heavy peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Eksigent
Column Packing: Reprosil C18, 3um
Column Dimensions: 75µm x 15cm
Flow Rate: 0.3 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	16.5	14	11.8	30.5	31.5	41.2	34.7	34.5	42.9	15	15	15
y6 (1+)	18.5	12.6	11.3	31.8	31.5	42	36.8	33.9	43.5	15	15	15
y5 (1+)	17.7	11.3	8.9	31.2	29.2	39	35.9	31.3	40	15	15	15
sum	17.7	11.9	10.7	31.1	30.8	40.7	35.8	33	42.1	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

View in CPTC Antibody Portal

Comments

Assay Details for CPTAC-6229 Collapse assay details

Data source: Panorama

Official Gene Symbol: MUTYH
Peptide Sequence: MGQQVLDNFFR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 7
Peptide Start: 521
Peptide End: 531

CPTAC ID: CPTAC-6229
Peptide Molecular Mass: 1,353.6500
Species: Homo Sapiens
Assay Type: Enrichment MRM
Enrichment Method: Peptide Immunoaffinity
Matrix: Frozen tissue
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich