SIGLEC1, CPTAC-6230 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
SIGLEC1	SN

Sequence Length (AA)	Molecular Weight (Da)
1709	182624

Protein Name
Sialoadhesin

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MGFLPKLLLL	ASFFPAGQAS	WGVSSPQDVQ	GVKGSCLLIP	CIFSFPADVE
60	70	80	90	100
VPDGITAIWY	YDYSGQRQVV	SHSADPKLVE	ARFRGRTEFM	GNPEHRVCNL
110	120	130	140	150
LLKDLQPEDS	GSYNFRFEIS	EVNRWSDVKG	TLVTVTEEPR	VPTIASPVEL
160	170	180	190	200
LEGTEVDFNC	STPYVCLQEQ	VRLQWQGQDP	ARSVTFNSQK	FEPTGVGHLE
210	220	230	240	250
TLHMAMSWQD	HGRILRCQLS	VANHRAQSEI	HLQVKYAPKG	VKILLSPSGR
260	270	280	290	300
NILPGELVTL	TCQVNSSYPA	VSSIKWLKDG	VRLQTKTGVL	HLPQAAWSDA
310	320	330	340	350
GVYTCQAENG	VGSLVSPPIS	LHIFMAEVQV	SPAGPILENQ	TVTLVCNTPN
360	370	380	390	400
EAPSDLRYSW	YKNHVLLEDA	HSHTLRLHLA	TRADTGFYFC	EVQNVHGSER
410	420	430	440	450
SGPVSVVVNH	PPLTPVLTAF	LETQAGLVGI	LHCSVVSEPL	ATLVLSHGGH
460	470	480	490	500
ILASTSGDSD	HSPRFSGTSG	PNSLRLEIRD	LEETDSGEYK	CSATNSLGNA
510	520	530	540	550
TSTLDFHANA	ARLLISPAAE	VVEGQAVTLS	CRSGLSPTPD	ARFSWYLNGA
560	570	580	590	600
LLHEGPGSSL	LLPAASSTDA	GSYHCRARDG	HSASGPSSPA	VLTVLYPPRQ
610	620	630	640	650
PTFTTRLDLD	AAGAGAGRRG	LLLCRVDSDP	PARLQLLHKD	RVVATSLPSG
660	670	680	690	700
GGCSTCGGCS	PRMKVTKAPN	LLRVEIHNPL	LEEEGLYLCE	ASNALGNAST
710	720	730	740	750
SATFNGQATV	LAIAPSHTLQ	EGTEANLTCN	VSREAAGSPA	NFSWFRNGVL
760	770	780	790	800
WAQGPLETVT	LLPVARTDAA	LYACRILTEA	GAQLSTPVLL	SVLYPPDRPK
810	820	830	840	850
LSALLDMGQG	HMALFICTVD	SRPLALLALF	HGEHLLATSL	GPQVPSHGRF
860	870	880	890	900
QAKAEANSLK	LEVRELGLGD	SGSYRCEATN	VLGSSNTSLF	FQVRGAWVQV
910	920	930	940	950
SPSPELQEGQ	AVVLSCQVHT	GVPEGTSYRW	YRDGQPLQES	TSATLRFAAI
960	970	980	990	1000
TLTQAGAYHC	QAQAPGSATT	SLAAPISLHV	SYAPRHVTLT	TLMDTGPGRL
1010	1020	1030	1040	1050
GLLLCRVDSD	PPAQLRLLHG	DRLVASTLQG	VGGPEGSSPR	LHVAVAPNTL
1060	1070	1080	1090	1100
RLEIHGAMLE	DEGVYICEAS	NTLGQASASA	DFDAQAVNVQ	VWPGATVREG
1110	1120	1130	1140	1150
QLVNLTCLVW	TTHPAQLTYT	WYQDGQQRLD	AHSIPLPNVT	VRDATSYRCG
1160	1170	1180	1190	1200
VGPPGRAPRL	SRPITLDVLY	APRNLRLTYL	LESHGGQLAL	VLCTVDSRPP
1210	1220	1230	1240	1250
AQLALSHAGR	LLASSTAASV	PNTLRLELRG	PQPRDEGFYS	CSARSPLGQA
1260	1270	1280	1290	1300
NTSLELRLEG	VRVILAPEAA	VPEGAPITVT	CADPAAHAPT	LYTWYHNGRW
1310	1320	1330	1340	1350
LQEGPAASLS	FLVATRAHAG	AYSCQAQDAQ	GTRSSRPAAL	QVLYAPQDAV
1360	1370	1380	1390	1400
LSSFRDSRAR	SMAVIQCTVD	SEPPAELALS	HDGKVLATSS	GVHSLASGTG
1410	1420	1430	1440	1450
HVQVARNALR	LQVQDVPAGD	DTYVCTAQNL	LGSISTIGRL	QVEGARVVAE
1460	1470	1480	1490	1500
PGLDVPEGAA	LNLSCRLLGG	PGPVGNSTFA	WFWNDRRLHA	EPVPTLAFTH
1510	1520	1530	1540	1550
VARAQAGMYH	CLAELPTGAA	ASAPVMLRVL	YPPKTPTMMV	FVEPEGGLRG
1560	1570	1580	1590	1600
ILDCRVDSEP	LASLTLHLGS	RLVASSQPQG	APAEPHIHVL	ASPNALRVDI
1610	1620	1630	1640	1650
EALRPSDQGE	YICSASNVLG	SASTSTYFGV	RALHRLHQFQ	QLLWVLGLLV
1660	1670	1680	1690	1700
GLLLLLLGLG	ACYTWRRRRV	CKQSMGENSV	EMAFQKETTQ	LIDPDAATCE
1709
TSTCAPPLG

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-6230 Collapse assay details

Data source: Panorama

Official Gene Symbol: SIGLEC1
Peptide Sequence: LHAEPVPTLAFTHVAR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 7
Peptide Start: 1488
Peptide End: 1503

CPTAC ID: CPTAC-6230
Peptide Molecular Mass: 1,757.9577
Species: Homo Sapiens
Assay Type: Enrichment MRM
Enrichment Method: Peptide Immunoaffinity
Matrix: Frozen tissue
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTRAP 5500
Internal Standard: Extended heavy peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Eksigent
Column Packing: Reprosil C18, 3um
Column Dimensions: 75µm x 15cm
Flow Rate: 0.3 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y10 (1+)	6.9	3.1	6.2	11.3	8.4	12	13.2	9	13.5	15	15	15
y7 (1+)	9.8	5.2	6.1	9.9	10.2	12.4	13.9	11.4	13.8	15	15	15
b6 (1+)	10.5	4.9	7.2	12.9	10.1	13	16.6	11.2	14.9	15	15	15
sum	7.1	3.4	5.5	9.9	9.2	12	12.2	9.8	13.2	15	15	15

SIGLEC1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-6230 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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SIGLEC1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-6230 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments