Overview Data source: UniProt

Official Gene Symbol Other Aliases
PRDX2 NKEFB,TDPX1
Sequence Length (AA) Molecular Weight (Da)
198 21892
Protein Name
Peroxiredoxin-2
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MASGNARIGK PAPDFKATAV VDGAFKEVKL SDYKGKYVVL FFYPLDFTFV
60 70 80 90 100
CPTEIIAFSN RAEDFRKLGC EVLGVSVDSQ FTHLAWINTP RKEGGLGPLN
110 120 130 140 150
IPLLADVTRR LSEDYGVLKT DEGIAYRGLF IIDGKGVLRQ ITVNDLPVGR
160 170 180 190 198
SVDEALRLVQ AFQYTDEHGE VCPAGWKPGS DTIKPNVDDS KEYFSKHN

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
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Assay Details for CPTAC-1447 Collapse assay details

Data source: Panorama

Official Gene Symbol
PRDX2
Peptide Sequence
ATAVVDGAFK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
17
Peptide End
26
CPTAC ID
CPTAC-1447
Peptide Molecular Mass
977.5182
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
depleted digested plasma
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva TSQ
Internal Standard
heavy stable isotope peptide (delta 8-10)
Peptide Standard Purity
>95%
Peptide Standard Label Type
15N
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 4.1 2.5 3 5.2 6.1 4 6.6 6.6 5 15 15 15
y8 (1+) 2.9 1.6 1.4 4.9 6 3.2 5.7 6.2 3.5 15 15 15
y6 (1+) 2.4 1.8 1.7 3.5 6.2 3.3 4.2 6.5 3.7 15 15 15
sum 1.9 1.7 1.1 3.8 6.1 3.1 4.2 6.3 3.3 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-1445 Collapse assay details

Data source: Panorama

Official Gene Symbol
PRDX2
Peptide Sequence
GLFIIDGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
128
Peptide End
135
CPTAC ID
CPTAC-1445
Peptide Molecular Mass
861.4960
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
depleted digested plasma
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva TSQ
Internal Standard
heavy stable isotope peptide (delta 8-10)
Peptide Standard Purity
>95%
Peptide Standard Label Type
15N
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 3.2 1.7 1.7 4.6 6 2.7 5.6 6.2 3.2 15 15 15
y6 (1+) 2 1.1 2.3 2.5 6.1 2.9 3.2 6.2 3.7 15 15 15
y3 (1+) 6.2 2.3 1.7 9.1 6.5 2.5 11 6.9 3 15 15 15
sum 1.6 1.3 1.7 3.8 6 2.4 4.1 6.1 2.9 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-1446 Collapse assay details

Data source: Panorama

Official Gene Symbol
PRDX2
Peptide Sequence
TDEGIAYR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
120
Peptide End
127
CPTAC ID
CPTAC-1446
Peptide Molecular Mass
923.4349
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
depleted digested plasma
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva TSQ
Internal Standard
heavy stable isotope peptide (delta 8-10)
Peptide Standard Purity
>95%
Peptide Standard Label Type
15N
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 3 2 2.2 4.9 5.5 3.8 5.7 5.9 4.4 15 15 15
y5 (1+) 3 1.9 1.3 4.7 5.1 3 5.6 5.4 3.3 15 15 15
y4 (1+) 2.9 2.3 2.6 4.5 6.1 3.5 5.4 6.5 4.4 15 15 15
sum 2.5 1.8 1.4 4.4 5.3 3.2 5.1 5.6 3.5 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-710 Collapse assay details

Data source: Panorama

Official Gene Symbol
PRDX2
Peptide Sequence
GLFIIDGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
128
Peptide End
135
CPTAC ID
CPTAC-710
Peptide Molecular Mass
861.4960
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
plasma
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Anti-peptide monoclonal antibodies generated for immuno-multiple reaction monitoring-mass spectrometry assays have a high probability of supporting Western blot and ELISA. Schoenherr RM, Saul RG, Whiteaker JR, Yan P, Whiteley GR, Paulovich AG. Mol Cell Proteomics. 2015 Feb;14(2):382-98. doi: 10.1074/mcp.O114.043133. Epub 2014 Dec 15. PMID: 25512614


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 Qtrap
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
15N
LC
Eksigent NanoLC 2D
Column Packing
ReproSil-Pur C18-AQ 3 um
Column Dimensions
100 mm x 75 um ID
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 48.1 16.7 7.5 46.8 29 10.2 67.1 33.5 12.7 15 15 15
y5 (1+) 19.2 5.6 2.7 24.2 7.5 5.1 30.9 9.4 5.8 15 15 15
y6 (1+) 25.8 6 3.9 26.9 6.6 4.4 37.3 8.9 5.9 15 15 15
sum 24.1 3.2 3.3 25.8 5.5 4.5 35.3 6.4 5.6 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-727 Collapse assay details

Data source: Panorama

Official Gene Symbol
PRDX2
Peptide Sequence
LSEDYGVLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
111
Peptide End
119
CPTAC ID
CPTAC-727
Peptide Molecular Mass
1,022.5284
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
plasma
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Anti-peptide monoclonal antibodies generated for immuno-multiple reaction monitoring-mass spectrometry assays have a high probability of supporting Western blot and ELISA. Schoenherr RM, Saul RG, Whiteaker JR, Yan P, Whiteley GR, Paulovich AG. Mol Cell Proteomics. 2015 Feb;14(2):382-98. doi: 10.1074/mcp.O114.043133. Epub 2014 Dec 15. PMID: 25512614


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 Qtrap
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Eksigent NanoLC 2D
Column Packing
ReproSil-Pur C18-AQ 3 um
Column Dimensions
100 mm x 75 um ID
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b7 (1+) 49 13.2 4.4 47.4 14.2 5.7 68.2 19.4 7.2 15 15 15
b3 (1+) 37.9 16.4 4.2 54.3 20.4 5.7 66.2 26.2 7.1 15 15 15
y6 (1+) 15.1 6.3 4.2 20.8 10.6 4.9 25.7 12.3 6.5 15 15 15
sum 20.8 5.8 3.5 23.7 9.4 4.4 31.5 11 5.6 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-1143 Collapse assay details

Data source: Panorama

Official Gene Symbol
PRDX2
Peptide Sequence
ATAVVDGAFK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
17
Peptide End
26
CPTAC ID
non-CPTAC-1143
Peptide Molecular Mass
977.5182
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
15N
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b4 (1+) 11.6 8.4 6.8 16.2 10 6.9 19.9 13.1 9.7 13 15 15
b3 (1+) 29 7.3 6.4 39.3 10.6 7.6 48.8 12.9 9.9 15 15 15
y5 (1+) 18.2 8.1 5.7 16.4 6 8 24.5 10.1 9.8 15 15 15
y6 (1+) 7.8 8.4 3.5 10.9 7.1 4.2 13.4 11 5.5 15 15 15
y8 (1+) 7.9 7.3 9 9.5 10.5 8 12.4 12.8 12 15 15 15
sum 4.2 4.5 4.8 7.1 5.6 4.4 8.2 7.2 6.5 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-1144 Collapse assay details

Data source: Panorama

Official Gene Symbol
PRDX2
Peptide Sequence
GLFIIDGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
128
Peptide End
135
CPTAC ID
non-CPTAC-1144
Peptide Molecular Mass
861.4960
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
15N
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 11.5 6.7 4.6 11.9 6.6 5.6 16.5 9.4 7.2 15 15 15
y3 (1+) 12 9.9 4.7 14.3 7.7 4.7 18.7 12.5 6.6 15 15 15
y4 (1+) 8.2 9.2 6.3 9 5.8 6.9 12.2 10.9 9.3 15 15 15
y5 (1+) 8.2 8.4 4.8 8.1 6.4 6.7 11.5 10.6 8.2 15 15 15
y6 (1+) 8.8 8.1 5.8 9.4 7.5 6.7 12.9 11 8.9 15 15 15
sum 4.5 6.3 3.2 5.8 5 3.9 7.3 8 5 15 15 15

Additional Resources and Comments