MAP2K6, CPTAC-821 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
MAP2K6	MEK6, MKK6, PRKMK6, SKK3

Sequence Length (AA)	Molecular Weight (Da)
334	37492

Protein Name
Dual specificity mitogen-activated protein kinase kinase 6

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSQSKGKKRN	PGLKIPKEAF	EQPQTSSTPP	RDLDSKACIS	IGNQNFEVKA
60	70	80	90	100
DDLEPIMELG	RGAYGVVEKM	RHVPSGQIMA	VKRIRATVNS	QEQKRLLMDL
110	120	130	140	150
DISMRTVDCP	FTVTFYGALF	REGDVWICME	LMDTSLDKFY	KQVIDKGQTI
160	170	180	190	200
PEDILGKIAV	SIVKALEHLH	SKLSVIHRDV	KPSNVLINAL	GQVKMCDFGI
210	220	230	240	250
SGYLVDSVAK	TIDAGCKPYM	APERINPELN	QKGYSVKSDI	WSLGITMIEL
260	270	280	290	300
AILRFPYDSW	GTPFQQLKQV	VEEPSPQLPA	DKFSAEFVDF	TSQCLKKNSK
310	320	330	334
ERPTYPELMQ	HPFFTLHESK	GTDVASFVKL	ILGD

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-820 Collapse assay details

Data source: Panorama

Official Gene Symbol: MAP2K6
Peptide Sequence: DVKPSNVLINALGQVK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 179
Peptide End: 194

CPTAC ID: CPTAC-820
Peptide Molecular Mass: 1,693.9727
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Pooled patient derived xenograft breast tumor digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TripleTOF 5600+ (SCIEX)
Internal Standard: Peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 2DPlus nano-LC (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 200 µm x 15 cm
Flow Rate: 800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y4 (1+)	10.9	7.5	9.6	13.2	8.2	11.5	17.1	11.1	15	15	15	15
y5 (1+)	12.4	8.5	9.1	23.9	7.8	10.9	26.9	11.5	14.2	15	15	15
y6 (1+)	25	14.1	13.6	24.7	14.7	12	35.1	20.4	18.1	15	15	15
y7 (1+)	15.8	11.6	10.1	16.8	16	10.2	23.1	19.8	14.4	15	15	15
y8 (1+)	20.9	11.8	8.2	22.3	10.3	8.2	30.6	15.7	11.6	15	15	15
b8 (1+)	26.2	15.8	17.8	33	16.5	17.2	42.1	22.8	24.8	15	15	15
b7 (1+)	13.8	8.1	6.4	17.2	10.2	5.4	22.1	13	8.4	15	15	15
b6 (1+)	17.3	9.4	8.6	16.4	13	8	23.8	16	11.7	15	15	15
b5 (1+)	26.7	17.8	19.1	26.5	23.4	23.1	37.6	29.4	30	15	15	15
sum	5.5	3.5	4.5	7.5	5.3	5.5	9.3	6.4	7.1	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for CPTAC-821 Collapse assay details

Data source: Panorama

Official Gene Symbol: MAP2K6
Peptide Sequence: GTDVASFVK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 321
Peptide End: 329

CPTAC ID: CPTAC-821
Peptide Molecular Mass: 922.4760
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Pooled patient derived xenograft breast tumor digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend