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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
MAP2K7 JNKK2, MEK7, MKK7, PRKMK7, SKK4
Sequence Length (AA) Molecular Weight (Da)
419 47485
Protein Name
Dual specificity mitogen-activated protein kinase kinase 7
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAASSLEQKL SRLEAKLKQE NREARRRIDL NLDISPQRPR PTLQLPLAND
60 70 80 90 100
GGSRSPSSES SPQHPTPPAR PRHMLGLPST LFTPRSMESI EIDQKLQEIM
110 120 130 140 150
KQTGYLTIGG QRYQAEINDL ENLGEMGSGT CGQVWKMRFR KTGHVIAVKQ
160 170 180 190 200
MRRSGNKEEN KRILMDLDVV LKSHDCPYIV QCFGTFITNT DVFIAMELMG
210 220 230 240 250
TCAEKLKKRM QGPIPERILG KMTVAIVKAL YYLKEKHGVI HRDVKPSNIL
260 270 280 290 300
LDERGQIKLC DFGISGRLVD SKAKTRSAGC AAYMAPERID PPDPTKPDYD
310 320 330 340 350
IRADVWSLGI SLVELATGQF PYKNCKTDFE VLTKVLQEEP PLLPGHMGFS
360 370 380 390 400
GDFQSFVKDC LTKDHRKRPK YNKLLEHSFI KRYETLEVDV ASWFKDVMAK
410 419
TESPRTSGVL SQPHLPFFR

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-3080 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP2K7
Peptide Sequence
DVKPSNILLDER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
243
Peptide End
254
CPTAC ID
CPTAC-3080
Peptide Molecular Mass
1,397.7514
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 5.7 3.6 6 9.7 5.4 6.1 11.3 6.5 8.6 15 15 15
y4 (1+) 7 4.2 4.8 10.1 6.1 4.8 12.3 7.4 6.8 15 15 15
b6 (1+) 6.4 4.1 6.2 9.1 6.3 6.7 11.1 7.5 9.1 15 15 15
sum 6.2 3.9 5.5 9.4 5.9 5.5 11.3 7.1 7.8 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-3079 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP2K7
Peptide Sequence
SPSSESSPQHPTPPARPR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
55
Peptide End
72
CPTAC ID
CPTAC-3079
Peptide Molecular Mass
1,913.9344
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (2+) 3.5 9.4 8.1 3.6 10.3 12.2 5 13.9 14.6 15 14 15
y8 (2+) 3.4 6.5 6.5 3.7 7.6 10 5 10 11.9 15 14 15
y13 (2+) 4.3 28.5 7.5 4.5 45.6 11.6 6.2 53.8 13.8 15 14 15
sum 2.9 7.2 7 3 8.2 11 4.2 10.9 13 15 14 15


Additional Resources and Comments


Assay Details for CPTAC-822 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP2K7
Peptide Sequence
TGHVIAVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
142
Peptide End
149
CPTAC ID
CPTAC-822
Peptide Molecular Mass
823.4916
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 19.4 14.2 10.3 19.7 13.2 12.6 27.6 19.4 16.3 15 15 15
y4 (1+) 11.4 8.6 5.9 14 8.9 7.8 18.1 12.4 9.8 15 15 15
y5 (1+) 9.4 7.9 6.1 9.9 7.9 8.4 13.7 11.2 10.4 15 15 15
b6 (1+) 10.6 9 7.9 14.7 8.4 6.6 18.1 12.3 10.3 15 15 15
b5 (1+) 14.6 6.6 10.1 17.9 4 10.8 23.1 7.7 14.8 15 15 15
b4 (1+) 13.9 9.4 6.6 12.2 9.7 7.8 18.5 13.5 10.2 15 15 15
b3 (1+) 7.1 5.6 3.9 8.2 6.4 4.1 10.8 8.5 5.7 15 15 15
sum 4.8 4.6 3 6.2 4.4 4.1 7.8 6.4 5.1 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-823 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP2K7
Peptide Sequence
TSGVLSQPHLPFFR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
406
Peptide End
419
CPTAC ID
CPTAC-823
Peptide Molecular Mass
1,584.8413
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 4.5 2.4 3.5 5.7 2.6 4.1 7.3 3.5 5.4 15 15 15
y5 (1+) 8.4 3.7 3.9 8.8 4.4 4.2 12.2 5.7 5.7 15 15 15
y6 (1+) 10.6 5.9 4 10.3 5.8 5.1 14.8 8.3 6.5 15 15 15
y7 (1+) 4.2 3.4 2.6 4.9 4.3 3.5 6.5 5.5 4.4 15 15 15
y9 (1+) 7.7 6.2 4.5 9.5 7 5.6 12.2 9.4 7.2 15 15 15
sum 2.7 1.7 1.9 3.8 2.4 2.9 4.7 2.9 3.5 15 15 15


Additional Resources and Comments