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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
MAPK11 PRKM11, SAPK2, SAPK2B
Sequence Length (AA) Molecular Weight (Da)
364 41357
Protein Name
Mitogen-activated protein kinase 11
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSGPRAGFYR QELNKTVWEV PQRLQGLRPV GSGAYGSVCS AYDARLRQKV
60 70 80 90 100
AVKKLSRPFQ SLIHARRTYR ELRLLKHLKH ENVIGLLDVF TPATSIEDFS
110 120 130 140 150
EVYLVTTLMG ADLNNIVKCQ ALSDEHVQFL VYQLLRGLKY IHSAGIIHRD
160 170 180 190 200
LKPSNVAVNE DCELRILDFG LARQADEEMT GYVATRWYRA PEIMLNWMHY
210 220 230 240 250
NQTVDIWSVG CIMAELLQGK ALFPGSDYID QLKRIMEVVG TPSPEVLAKI
260 270 280 290 300
SSEHARTYIQ SLPPMPQKDL SSIFRGANPL AIDLLGRMLV LDSDQRVSAA
310 320 330 340 350
EALAHAYFSQ YHDPEDEPEA EPYDESVEAK ERTLEEWKEL TYQEVLSFKP
360 364
PEPPKPPGSL EIEQ

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-2998 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK11
Peptide Sequence
ALFPGSDYIDQLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
221
Peptide End
233
CPTAC ID
CPTAC-2998
Peptide Molecular Mass
1,465.7453
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 7 4.5 4.2 6.9 7 5.8 9.8 8.3 7.2 15 15 15
y10 (1+) 7.2 5 5.7 6.6 8.5 8 9.8 9.9 9.8 15 15 15
y9 (1+) 6.3 4.5 4.9 7 8.5 7.2 9.4 9.6 8.7 15 15 15
sum 6.8 4.7 5.2 6.3 8.2 7.4 9.3 9.5 9 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-872 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK11
Peptide Sequence
DLSSIFR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
269
Peptide End
275
CPTAC ID
CPTAC-872
Peptide Molecular Mass
836.4392
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 9.3 10.4 5.6 11.5 10.4 5.9 14.8 14.7 8.1 15 15 15
y4 (1+) 6.8 6.1 4.6 11.2 9.2 6.7 13.1 11 8.1 15 15 15
y5 (1+) 4.9 3.4 3 7.8 5.5 5.4 9.2 6.5 6.2 15 15 15
sum 3.8 3.6 2.7 7.6 5.9 5.2 8.5 6.9 5.9 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-873 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK11
Peptide Sequence
GANPLAIDLLGR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
276
Peptide End
287
CPTAC ID
CPTAC-873
Peptide Molecular Mass
1,208.6877
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 5.5 2.8 5 9.4 6.9 6.8 10.9 7.4 8.4 15 15 15
y5 (1+) 6.8 5.4 5.7 8.1 7.7 7.3 10.6 9.4 9.3 15 15 15
y6 (1+) 4 5.2 5.3 8.1 6 5.2 9 7.9 7.4 15 15 15
y7 (1+) 6.3 2.6 5.1 7.2 5.3 4.9 9.6 5.9 7.1 15 15 15
y8 (1+) 4.6 4.1 5.3 10.6 7.5 5.9 11.6 8.5 7.9 15 15 15
y9 (1+) 5.2 3.4 4.2 6.9 6.4 5.5 8.6 7.2 6.9 15 15 15
sum 3.9 2.1 3.2 6.2 5.3 4.7 7.3 5.7 5.7 15 15 15


Additional Resources and Comments