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Overview Data source: UniProt

Official Gene Symbol Other Aliases
MAPK12 ERK6, SAPK3
Sequence Length (AA) Molecular Weight (Da)
367 41940
Protein Name
Mitogen-activated protein kinase 12
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSSPPPARSG FYRQEVTKTA WEVRAVYRDL QPVGSGAYGA VCSAVDGRTG
60 70 80 90 100
AKVAIKKLYR PFQSELFAKR AYRELRLLKH MRHENVIGLL DVFTPDETLD
110 120 130 140 150
DFTDFYLVMP FMGTDLGKLM KHEKLGEDRI QFLVYQMLKG LRYIHAAGII
160 170 180 190 200
HRDLKPGNLA VNEDCELKIL DFGLARQADS EMTGYVVTRW YRAPEVILNW
210 220 230 240 250
MRYTQTVDIW SVGCIMAEMI TGKTLFKGSD HLDQLKEIMK VTGTPPAEFV
260 270 280 290 300
QRLQSDEAKN YMKGLPELEK KDFASILTNA SPLAVNLLEK MLVLDAEQRV
310 320 330 340 350
TAGEALAHPY FESLHDTEDE PQVQKYDDSF DDVDRTLDEW KRVTYKEVLS
360 367
FKPPRQLGAR VSKETPL

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-874 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK12
Peptide Modified Sequence
DLQPVGSGAYGAVC[+57.0]SAVDGR
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
42
Peptide - Site of Modification
14
Peptide Start
29
Peptide End
48
CPTAC ID
CPTAC-874
Peptide Molecular Mass
1,977.9214
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 75.3 47.9 13.9 132.3 36.8 18.6 152.2 60.4 23.2 15 15 15
y4 (1+) 86.6 39.5 32.4 160.9 47.3 24.3 182.7 61.6 40.5 15 15 15
y5 (1+) 85.1 21.5 23.8 162.7 22.4 21.9 183.6 31 32.3 15 15 15
y6 (1+) 73.2 16.4 15.2 161.5 12.4 16.2 177.3 20.6 22.2 14 15 15
y7 (1+) 85.7 12.8 14.5 175.1 17.7 17.3 194.9 21.8 22.6 15 15 15
y8 (1+) 108.5 33.8 18 126.3 40.1 19.5 166.5 52.4 26.5 14 15 15
y9 (1+) 87.2 29.7 30.5 110.7 36.5 37.2 140.9 47.1 48.1 14 15 15
y10 (1+) 73.7 40.2 23.9 164.3 42.3 27.9 180.1 58.4 36.7 15 15 15
y11 (1+) 71.8 26.1 29.6 109.1 32.4 29.4 130.6 41.6 41.7 13 15 15
y12 (1+) 74.5 56 41.4 103.5 77.5 48.5 127.5 95.6 63.8 12 15 15
y13 (1+) 47.6 49.2 46.3 64.3 51 71.4 80 70.9 85.1 9 15 15
y14 (1+) 139.5 67.4 57.2 150 77.4 40.7 204.8 102.6 70.2 9 15 15
y15 (1+) 30.8 38.6 28.1 51.1 44.1 28.5 59.7 58.6 40 9 15 15
b15 (1+) NA NA NA NA NA NA NA NA NA 3 4 6
b14 (1+) 149.4 131.7 NA 145.3 122.6 NA 208.4 179.9 NA 11 9 4
b13 (1+) 138.4 119.5 120.1 140.9 146.3 123.5 197.5 188.9 172.3 10 12 12
b12 (1+) 124.9 77.2 42.2 125.1 96.1 44.3 176.8 123.3 61.2 10 15 15
b11 (1+) 85.7 70.7 89.8 103.9 85.7 87.1 134.7 111.1 125.1 14 15 15
b10 (1+) 72.3 75.6 57.1 126.8 74.7 66.8 146 106.3 87.9 14 14 15
b9 (1+) 87.6 27.5 40.1 116.3 53.7 47.8 145.6 60.3 62.4 14 15 15
b8 (1+) 70.9 51.8 80.9 113.3 49.9 114.4 133.7 71.9 140.1 14 15 15
b7 (1+) 93.4 81.9 50.9 131.5 138.1 70 161.3 160.6 86.5 15 15 15
b6 (1+) 101.8 65.7 54.9 134.3 74.2 105.9 168.5 99.1 119.3 13 15 15
b5 (1+) 107.7 62.9 69.1 174.7 84.2 86 205.2 105.1 110.3 15 14 15
b4 (1+) 89.3 42.8 68.3 97.1 62.9 80.1 131.9 76.1 105.3 15 15 15
b3 (1+) 79.9 51.5 31.9 102.9 48.4 36 130.3 70.7 48.1 15 15 15
sum 58.2 10.4 4.8 126.3 11.1 4.7 139.1 15.2 6.7 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-875 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK12
Peptide Sequence
QADSEMTGYVVTR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
177
Peptide End
189
CPTAC ID
CPTAC-875
Peptide Molecular Mass
1,455.6664
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 19.2 13.9 9.2 19.7 14.9 8.5 27.5 20.4 12.5 15 15 15
y7 (1+) 21.9 7.4 8.3 20 7.4 9.9 29.7 10.5 12.9 15 15 15
y8 (1+) 19.6 8.2 9.6 17.8 8.1 10.1 26.5 11.5 13.9 15 15 15
y10 (1+) 29 11.6 10.8 24.7 10.3 9.4 38.1 15.5 14.3 15 15 15
y11 (1+) 26.7 12.7 7.9 22.4 13.9 10.4 34.9 18.8 13.1 15 15 15
sum 7.6 5.4 3.6 9.6 6.2 4.7 12.2 8.2 5.9 15 15 15

Additional Resources and Comments