MAPK12, CPTAC-2914 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
MAPK12	ERK6, SAPK3

Sequence Length (AA)	Molecular Weight (Da)
367	41940

Protein Name
Mitogen-activated protein kinase 12

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSSPPPARSG	FYRQEVTKTA	WEVRAVYRDL	QPVGSGAYGA	VCSAVDGRTG
60	70	80	90	100
AKVAIKKLYR	PFQSELFAKR	AYRELRLLKH	MRHENVIGLL	DVFTPDETLD
110	120	130	140	150
DFTDFYLVMP	FMGTDLGKLM	KHEKLGEDRI	QFLVYQMLKG	LRYIHAAGII
160	170	180	190	200
HRDLKPGNLA	VNEDCELKIL	DFGLARQADS	EMTGYVVTRW	YRAPEVILNW
210	220	230	240	250
MRYTQTVDIW	SVGCIMAEMI	TGKTLFKGSD	HLDQLKEIMK	VTGTPPAEFV
260	270	280	290	300
QRLQSDEAKN	YMKGLPELEK	KDFASILTNA	SPLAVNLLEK	MLVLDAEQRV
310	320	330	340	350
TAGEALAHPY	FESLHDTEDE	PQVQKYDDSF	DDVDRTLDEW	KRVTYKEVLS
360	367
FKPPRQLGAR	VSKETPL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-874 Collapse assay details

Data source: Panorama

Official Gene Symbol: MAPK12
Peptide Modified Sequence: DLQPVGSGAYGAVC[+57.0]SAVDGR
Modification Type: Carbamidomethyl Cysteine
Protein - Site of Modification: 42
Peptide - Site of Modification: 14
Peptide Start: 29
Peptide End: 48

CPTAC ID: CPTAC-874
Peptide Molecular Mass: 1,977.9214
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Pooled patient derived xenograft breast tumor digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TripleTOF 5600+ (SCIEX)
Internal Standard: Peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 2DPlus nano-LC (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 200 µm x 15 cm
Flow Rate: 800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	75.3	47.9	13.9	132.3	36.8	18.6	152.2	60.4	23.2	15	15	15
y4 (1+)	86.6	39.5	32.4	160.9	47.3	24.3	182.7	61.6	40.5	15	15	15
y5 (1+)	85.1	21.5	23.8	162.7	22.4	21.9	183.6	31	32.3	15	15	15
y6 (1+)	73.2	16.4	15.2	161.5	12.4	16.2	177.3	20.6	22.2	14	15	15
y7 (1+)	85.7	12.8	14.5	175.1	17.7	17.3	194.9	21.8	22.6	15	15	15
y8 (1+)	108.5	33.8	18	126.3	40.1	19.5	166.5	52.4	26.5	14	15	15
y9 (1+)	87.2	29.7	30.5	110.7	36.5	37.2	140.9	47.1	48.1	14	15	15
y10 (1+)	73.7	40.2	23.9	164.3	42.3	27.9	180.1	58.4	36.7	15	15	15
y11 (1+)	71.8	26.1	29.6	109.1	32.4	29.4	130.6	41.6	41.7	13	15	15
y12 (1+)	74.5	56	41.4	103.5	77.5	48.5	127.5	95.6	63.8	12	15	15
y13 (1+)	47.6	49.2	46.3	64.3	51	71.4	80	70.9	85.1	9	15	15
y14 (1+)	139.5	67.4	57.2	150	77.4	40.7	204.8	102.6	70.2	9	15	15
y15 (1+)	30.8	38.6	28.1	51.1	44.1	28.5	59.7	58.6	40	9	15	15
b15 (1+)	NA	NA	NA	NA	NA	NA	NA	NA	NA	3	4	6
b14 (1+)	149.4	131.7	NA	145.3	122.6	NA	208.4	179.9	NA	11	9	4
b13 (1+)	138.4	119.5	120.1	140.9	146.3	123.5	197.5	188.9	172.3	10	12	12
b12 (1+)	124.9	77.2	42.2	125.1	96.1	44.3	176.8	123.3	61.2	10	15	15
b11 (1+)	85.7	70.7	89.8	103.9	85.7	87.1	134.7	111.1	125.1	14	15	15
b10 (1+)	72.3	75.6	57.1	126.8	74.7	66.8	146	106.3	87.9	14	14	15
b9 (1+)	87.6	27.5	40.1	116.3	53.7	47.8	145.6	60.3	62.4	14	15	15
b8 (1+)	70.9	51.8	80.9	113.3	49.9	114.4	133.7	71.9	140.1	14	15	15
b7 (1+)	93.4	81.9	50.9	131.5	138.1	70	161.3	160.6	86.5	15	15	15
b6 (1+)	101.8	65.7	54.9	134.3	74.2	105.9	168.5	99.1	119.3	13	15	15
b5 (1+)	107.7	62.9	69.1	174.7	84.2	86	205.2	105.1	110.3	15	14	15
b4 (1+)	89.3	42.8	68.3	97.1	62.9	80.1	131.9	76.1	105.3	15	15	15
b3 (1+)	79.9	51.5	31.9	102.9	48.4	36	130.3	70.7	48.1	15	15	15
sum	58.2	10.4	4.8	126.3	11.1	4.7	139.1	15.2	6.7	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for CPTAC-2914 Collapse assay details

Data source: Panorama

Official Gene Symbol: MAPK12
Peptide Sequence: LYRPFQSELFAK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 58
Peptide End: 69

CPTAC ID: CPTAC-2914
Peptide Molecular Mass: 1,497.7980
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend