MAPK10, CPTAC-2915 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
MAPK10	JNK3, JNK3A, PRKM10, SAPK1B

Sequence Length (AA)	Molecular Weight (Da)
464	52585

Protein Name
Mitogen-activated protein kinase 10

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSLHFLYYCS	EPTLDVKIAF	CQGFDKQVDV	SYIAKHYNMS	KSKVDNQFYS
60	70	80	90	100
VEVGDSTFTV	LKRYQNLKPI	GSGAQGIVCA	AYDAVLDRNV	AIKKLSRPFQ
110	120	130	140	150
NQTHAKRAYR	ELVLMKCVNH	KNIISLLNVF	TPQKTLEEFQ	DVYLVMELMD
160	170	180	190	200
ANLCQVIQME	LDHERMSYLL	YQMLCGIKHL	HSAGIIHRDL	KPSNIVVKSD
210	220	230	240	250
CTLKILDFGL	ARTAGTSFMM	TPYVVTRYYR	APEVILGMGY	KENVDIWSVG
260	270	280	290	300
CIMGEMVRHK	ILFPGRDYID	QWNKVIEQLG	TPCPEFMKKL	QPTVRNYVEN
310	320	330	340	350
RPKYAGLTFP	KLFPDSLFPA	DSEHNKLKAS	QARDLLSKML	VIDPAKRISV
360	370	380	390	400
DDALQHPYIN	VWYDPAEVEA	PPPQIYDKQL	DEREHTIEEW	KELIYKEVMN
410	420	430	440	450
SEEKTKNGVV	KGQPSPSGAA	VNSSESLPPS	SSVNDISSMS	TDQTLASDTD
460	464
SSLEASAGPL	GCCR

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-2916 Collapse assay details

Data source: Panorama

Official Gene Symbol: MAPK10
Peptide Sequence: LFPDSLFPADSEHNK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 312
Peptide End: 326

CPTAC ID: CPTAC-2916
Peptide Molecular Mass: 1,715.8155
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (1+)	9.6	8.2	6.4	8.8	8.9	9.1	13	12.1	11.1	15	15	15
y6 (1+)	13.6	8.7	5.1	14	9.2	8.5	19.5	12.7	9.9	15	15	15
b5 (1+)	9	8.1	6	8.9	8.7	8.5	12.7	11.9	10.4	15	15	15
sum	9.6	8.2	6	8.9	8.8	8.8	13.1	12	10.7	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for CPTAC-2915 Collapse assay details

Data source: Panorama

Official Gene Symbol: MAPK10
Peptide Sequence: VDNQFYSVEVGDSTFTVLK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 44
Peptide End: 62

CPTAC ID: CPTAC-2915
Peptide Molecular Mass: 2,147.0423
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend