AHSG, CPTAC-1066 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
AHSG	FETUA

Sequence Length (AA)	Molecular Weight (Da)
367	39325

Protein Name
Alpha-2-HS-glycoprotein

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MKSLVLLLCL	AQLWGCHSAP	HGPGLIYRQP	NCDDPETEEA	ALVAIDYINQ
60	70	80	90	100
NLPWGYKHTL	NQIDEVKVWP	QQPSGELFEI	EIDTLETTCH	VLDPTPVARC
110	120	130	140	150
SVRQLKEHAV	EGDCDFQLLK	LDGKFSVVYA	KCDSSPDSAE	DVRKVCQDCP
160	170	180	190	200
LLAPLNDTRV	VHAAKAALAA	FNAQNNGSNF	QLEEISRAQL	VPLPPSTYVE
210	220	230	240	250
FTVSGTDCVA	KEATEAAKCN	LLAEKQYGFC	KATLSEKLGG	AEVAVTCTVF
260	270	280	290	300
QTQPVTSQPQ	PEGANEAVPT	PVVDPDAPPS	PPLGAPGLPP	AGSPPDSHVL
310	320	330	340	350
LAAPPGHQLH	RAHYDLRHTF	MGVVSLGSPS	GEVSHPRKTR	TVVQPSVGAA
360	367
AGPVVPPCPG	RIRHFKV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-1067 Collapse assay details

Data source: Panorama

Official Gene Symbol: AHSG
Peptide Sequence: APHGPGLIYR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 19
Peptide End: 28

CPTAC ID: non-CPTAC-1067
Peptide Molecular Mass: 1,079.5876
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 6490 Triple Quad (Agilent)
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b4 (1+)	19.5	10.9	12.4	29.4	12.7	11.4	35.3	16.7	16.8	11	15	15
b3 (1+)	23	10.7	9	24.5	10.5	8.9	33.6	15	12.7	15	15	15
y9 (2+)	22.3	12	9.6	19.9	13.2	9.9	29.9	17.8	13.8	14	15	15
y6 (1+)	14.5	7.2	3.7	14.8	9.1	6.6	20.7	11.6	7.6	15	15	15
y7 (1+)	12.1	6.9	6	9.8	6.8	6.2	15.6	9.7	8.6	15	15	15
sum	5.6	5.4	3.3	6.5	5.3	3.8	8.6	7.6	5	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-3578 Collapse assay details

Data source: Panorama

Official Gene Symbol: Ahsg
Peptide Sequence: EVTDPAK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 212
Peptide End: 218

CPTAC ID: non-CPTAC-3578
Peptide Molecular Mass: 758.3810
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers