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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

AHSG

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
AHSG FETUA
Sequence Length (AA) Molecular Weight (Da)
367 39325
Protein Name
Alpha-2-HS-glycoprotein
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MKSLVLLLCL AQLWGCHSAP HGPGLIYRQP NCDDPETEEA ALVAIDYINQ
60 70 80 90 100
NLPWGYKHTL NQIDEVKVWP QQPSGELFEI EIDTLETTCH VLDPTPVARC
110 120 130 140 150
SVRQLKEHAV EGDCDFQLLK LDGKFSVVYA KCDSSPDSAE DVRKVCQDCP
160 170 180 190 200
LLAPLNDTRV VHAAKAALAA FNAQNNGSNF QLEEISRAQL VPLPPSTYVE
210 220 230 240 250
FTVSGTDCVA KEATEAAKCN LLAEKQYGFC KATLSEKLGG AEVAVTCTVF
260 270 280 290 300
QTQPVTSQPQ PEGANEAVPT PVVDPDAPPS PPLGAPGLPP AGSPPDSHVL
310 320 330 340 350
LAAPPGHQLH RAHYDLRHTF MGVVSLGSPS GEVSHPRKTR TVVQPSVGAA
360 367
AGPVVPPCPG RIRHFKV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-1067 Collapse assay details

Data source: Panorama

Official Gene Symbol
AHSG
Peptide Sequence
APHGPGLIYR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
19
Peptide End
28
CPTAC ID
non-CPTAC-1067
Peptide Molecular Mass
1,079.5876
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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 LOD (fmol)LLOQ (fmol)
PeptideTransitionblank onlyblank+low-concrsd limitblank onlyblank+low-concrsd limit
APHGPGLIYR2.b3.14.8e+04.8e+03.1e+144.8e+04.9e+09.4e+14
2.b4.19.5e+0NA1.1e+79.6e+0NA3.4e+7
2.y6.11.3e+01.3e+01.3e+01.3e+01.4e+01.4e+0
2.y7.18.6e-18.5e-18.3e-18.8e-19.0e-18.3e-1
2.y9.26.7e+0NA1.1e+76.8e+0NA3.3e+7
Sum2.0e+02.0e+02.1e+02.2e+02.2e+02.4e+0
 Curve Fit
PeptideTransitionslopeinterceptr squared
APHGPGLIYR2.y9.24.75-31.31
2.y7.14.83-4.021
2.y6.14.79-6.221
2.b4.15.34-49.971
2.b3.15.18-24.251
Sum4.6-8.831

Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b4 (1+) 19.5 10.9 12.4 29.4 12.7 11.4 35.3 16.7 16.8 11 15 15
b3 (1+) 23 10.7 9 24.5 10.5 8.9 33.6 15 12.7 15 15 15
y9 (2+) 22.3 12 9.6 19.9 13.2 9.9 29.9 17.8 13.8 14 15 15
y6 (1+) 14.5 7.2 3.7 14.8 9.1 6.6 20.7 11.6 7.6 15 15 15
y7 (1+) 12.1 6.9 6 9.8 6.8 6.2 15.6 9.7 8.6 15 15 15
sum 5.6 5.4 3.3 6.5 5.3 3.8 8.6 7.6 5 15 15 15

Additional Resources and Comments

Resources
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View Peptide in Panorama
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Comments

Assay Details for non-CPTAC-3578 Collapse assay details

Data source: Panorama

Official Gene Symbol
Ahsg
Peptide Sequence
EVTDPAK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
212
Peptide End
218
CPTAC ID
non-CPTAC-3578
Peptide Molecular Mass
758.3810
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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 LOD (fmol per injection(20ul))LLOQ (fmol per injection(20ul))
PeptideTransitionblank onlyblank+low-concrsd limitblank onlyblank+low-concrsd limit
EVTDPAK2.y2.1-6.4e+07.3e+0-1.3e+1-1.6e+04.8e+1-1.2e+1
2.y3.18.7e+08.9e+08.6e+08.8e+09.5e+08.6e+0
2.y4.11.6e+11.7e+11.6e+11.6e+11.8e+11.6e+1
2.y5.16.3e+06.5e+06.2e+06.5e+06.9e+06.2e+0
2.y5.21.6e+11.6e+11.6e+11.6e+11.7e+11.7e+1
Sum1.1e+11.2e+11.1e+11.1e+11.2e+11.1e+1
 Curve Fit
PeptideTransitionslopeinterceptr squared
EVTDPAK2.y5.20.14-2.261
2.y5.10.12-0.771
2.y4.10.12-21
2.y3.10.13-1.11
2.y2.10.010.091
Sum0.13-1.471

Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 9.2 7 7.8 31.8 16.7 9.1 33.1 18.1 12 15 15 15
y5 (1+) 8.2 5.6 5.5 35.3 9.9 10.2 36.2 11.4 11.6 15 15 15
y5 (2+) 9.6 4.2 9.2 14.7 7.5 11.9 17.6 8.6 15 9 9 9
y2 (1+) 23.2 9.1 5.3 47.8 21.1 7 53.1 23 8.8 15 15 15
y3 (1+) 7 5.9 4.4 39 10 14 39.6 11.6 14.7 15 15 15
sum 5.3 4.4 3.1 36.1 9.7 8.9 36.5 10.7 9.4 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-1066 Collapse assay details

Data source: Panorama

Official Gene Symbol
AHSG
Peptide Sequence
FSVVYAK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
125
Peptide End
131
CPTAC ID
non-CPTAC-1066
Peptide Molecular Mass
812.4432
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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 LOD (fmol)LLOQ (fmol)
PeptideTransitionblank onlyblank+low-concrsd limitblank onlyblank+low-concrsd limit
FSVVYAK2.b2.17.3e-17.3e-17.3e-17.4e-17.4e-17.3e-1
2.y2.12.1e+02.1e+02.2e+02.2e+02.2e+02.2e+0
2.y3.11.9e+01.9e+01.9e+01.9e+01.9e+01.9e+0
2.y5.14.8e-14.8e-14.8e-14.8e-14.8e-14.8e-1
2.y6.16.2e-16.2e-16.2e-16.2e-16.3e-16.2e-1
Sum9.8e-19.8e-19.8e-19.9e-19.9e-19.8e-1
 Curve Fit
PeptideTransitionslopeinterceptr squared
FSVVYAK2.y6.11.33-0.831
2.y5.11.2-0.571
2.y3.11.18-2.221
2.y2.11.13-2.421
2.b2.11.2-0.881
Sum1.23-1.211

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 6.1 2.5 3.2 14.5 4.6 4.1 15.7 5.2 5.2 15 15 15
y2 (1+) 7.5 5.7 5 11 6.6 5.5 13.3 8.7 7.4 15 15 15
y3 (1+) 11.4 4.9 4.3 11.3 11 6.4 16.1 12 7.7 15 15 15
y5 (1+) 7.7 7.3 3.6 12.3 6.7 4 14.5 9.9 5.4 15 15 15
y6 (1+) 8.8 6.6 5.2 15.5 8.6 5.2 17.8 10.8 7.4 15 15 15
sum 2.3 2.8 2 9.8 5.4 3 10.1 6.1 3.6 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments
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