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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

SERPINF2

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
SERPINF2 AAP, PLI
Sequence Length (AA) Molecular Weight (Da)
491 54566
Protein Name
Alpha-2-antiplasmin
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MALLWGLLVL SWSCLQGPCS VFSPVSAMEP LGRQLTSGPN QEQVSPLTLL
60 70 80 90 100
KLGNQEPGGQ TALKSPPGVC SRDPTPEQTH RLARAMMAFT ADLFSLVAQT
110 120 130 140 150
STCPNLILSP LSVALALSHL ALGAQNHTLQ RLQQVLHAGS GPCLPHLLSR
160 170 180 190 200
LCQDLGPGAF RLAARMYLQK GFPIKEDFLE QSEQLFGAKP VSLTGKQEDD
210 220 230 240 250
LANINQWVKE ATEGKIQEFL SGLPEDTVLL LLNAIHFQGF WRNKFDPSLT
260 270 280 290 300
QRDSFHLDEQ FTVPVEMMQA RTYPLRWFLL EQPEIQVAHF PFKNNMSFVV
310 320 330 340 350
LVPTHFEWNV SQVLANLSWD TLHPPLVWER PTKVRLPKLY LKHQMDLVAT
360 370 380 390 400
LSQLGLQELF QAPDLRGISE QSLVVSGVQH QSTLELSEVG VEAAAATSIA
410 420 430 440 450
MSRMSLSSFS VNRPFLFFIF EDTTGLPLFV GSVRNPNPSA PRELKEQQDS
460 470 480 490 491
PGNKDFLQSL KGFPRGDKLF GPDLKLVPPM EEDYPQFGSP K

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-1065 Collapse assay details

Data source: Panorama

Official Gene Symbol
SERPINF2
Peptide Sequence
LGNQEPGGQTALK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
52
Peptide End
64
CPTAC ID
non-CPTAC-1065
Peptide Molecular Mass
1,311.6783
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b5 (1+) 24.5 7.6 4.6 21.1 12.9 5.8 32.3 15 7.4 14 15 15
b4 (1+) 26.4 20 9.3 36 21.7 8.5 44.6 29.5 12.6 11 15 15
y8 (1+) 12.9 6.7 7 17.8 7.4 7 22 10 9.9 14 15 15
sum 10.5 5.6 5.8 14.2 6.2 5.9 17.7 8.4 8.3 15 15 15

Additional Resources and Comments

Resources
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Assay Details for non-CPTAC-5573 Collapse assay details

Data source: Panorama

Official Gene Symbol
Serpinf2
Peptide Sequence
LPLPALFK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
44
Peptide End
51
CPTAC ID
non-CPTAC-5573
Peptide Molecular Mass
897.5688
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b4 (2+) 4.5 2.2 3.2 13.4 5.6 5.7 14.1 6 6.5 15 15 15
y7 (2+) 2 3.2 3.4 13.1 5.7 5.9 13.3 6.5 6.8 15 15 15
y5 (1+) 2.6 3.6 2.1 14 5 5.4 14.2 6.2 5.8 15 15 15
y6 (1+) 6.2 4.5 2.8 16.2 4.2 6.6 17.3 6.2 7.2 15 15 15
y7 (1+) 5 4 3.1 18.3 10.2 6 19 11 6.8 15 15 15
sum 1.1 1.7 1.6 13.6 4.9 5.1 13.6 5.2 5.3 15 15 15

Additional Resources and Comments

Resources
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View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-3405 Collapse assay details

Data source: Panorama

Official Gene Symbol
Serpinf2
Peptide Sequence
NPNPSALPQLQEQR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
435
Peptide End
448
CPTAC ID
non-CPTAC-3405
Peptide Molecular Mass
1,590.8114
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 6.5 4.8 5.6 17.1 8.7 6.5 18.3 9.9 8.6 15 15 15
y12 (2+) 7.5 6.1 10.8 14.3 9.6 11.3 16.1 11.4 15.6 15 15 15
y11 (2+) 5.7 4.5 7.1 12.7 7.4 6.8 13.9 8.7 9.8 15 15 15
b2 (1+) 9.2 5.7 6.8 16.8 6.9 7.5 19.2 8.9 10.1 15 15 15
b3 (1+) 10.4 8 5.7 11.8 11.2 9.7 15.7 13.8 11.3 15 15 15
sum 5.3 3 3 14.9 7.1 5.3 15.8 7.7 6.1 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments