Show Nav →
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Serpinf2

Previous Assay Next Assay
Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Serpinf2 Pli
Sequence Length (AA) Molecular Weight (Da)
491 54972
Protein Name
Alpha-2-antiplasmin
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MALLRGLLVL SLSCLQGPCF TFSPVSAVDL PGQQPVSEQA QQKLPLPALF
60 70 80 90 100
KLDNQDFGDH ATLKRSPGHC KSVPTAEETR RLAQAMMAFT TDLFSLVAQT
110 120 130 140 150
STSSNLVLSP LSVALALSHL ALGAQNQTLH SLHRVLHMNT GSCLPHLLSH
160 170 180 190 200
FYQNLGPGTI RLAARIYLQK GFPIKDDFLE QSERLFGAKP VKLTGKQEED
210 220 230 240 250
LANINQWVKE ATEGKIEDFL SELPDSTVLL LLNAIHFHGF WRTKFDPSLT
260 270 280 290 300
QKDFFHLDER FTVSVDMMHA VSYPLRWFLL EQPEIQVAHF PFKNNMSFVV
310 320 330 340 350
VMPTYFEWNV SEVLANLTWD TLYHPSLQER PTKVWLPKLH LQQQLDLVAT
360 370 380 390 400
LSQLGLQELF QGPDLRGISE QNLVVSSVQH QSTMELSEAG VEAAAATSVA
410 420 430 440 450
MNRMSLSSFT VNRPFLFFIM EDTIGVPLFV GSVRNPNPSA LPQLQEQRDS
460 470 480 490 491
PDNRLIGQND KADFHGGKTF GPDLKLAPRM EEDYPQFSSP K

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for non-CPTAC-1065 Collapse assay details

Data source: Panorama

Official Gene Symbol
SERPINF2
Peptide Sequence
LGNQEPGGQTALK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
52
Peptide End
64
CPTAC ID
non-CPTAC-1065
Peptide Molecular Mass
1,311.6783
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b5 (1+) 24.5 7.6 4.6 21.1 12.9 5.8 32.3 15 7.4 14 15 15
b4 (1+) 26.4 20 9.3 36 21.7 8.5 44.6 29.5 12.6 11 15 15
y8 (1+) 12.9 6.7 7 17.8 7.4 7 22 10 9.9 14 15 15
sum 10.5 5.6 5.8 14.2 6.2 5.9 17.7 8.4 8.3 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-5573 Collapse assay details

Data source: Panorama

Official Gene Symbol
Serpinf2
Peptide Sequence
LPLPALFK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
44
Peptide End
51
CPTAC ID
non-CPTAC-5573
Peptide Molecular Mass
897.5688
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b4 (2+) 4.5 2.2 3.2 13.4 5.6 5.7 14.1 6 6.5 15 15 15
y7 (2+) 2 3.2 3.4 13.1 5.7 5.9 13.3 6.5 6.8 15 15 15
y5 (1+) 2.6 3.6 2.1 14 5 5.4 14.2 6.2 5.8 15 15 15
y6 (1+) 6.2 4.5 2.8 16.2 4.2 6.6 17.3 6.2 7.2 15 15 15
y7 (1+) 5 4 3.1 18.3 10.2 6 19 11 6.8 15 15 15
sum 1.1 1.7 1.6 13.6 4.9 5.1 13.6 5.2 5.3 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-3405 Collapse assay details

Data source: Panorama

Official Gene Symbol
Serpinf2
Peptide Sequence
NPNPSALPQLQEQR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
435
Peptide End
448
CPTAC ID
non-CPTAC-3405
Peptide Molecular Mass
1,590.8114
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 6.5 4.8 5.6 17.1 8.7 6.5 18.3 9.9 8.6 15 15 15
y12 (2+) 7.5 6.1 10.8 14.3 9.6 11.3 16.1 11.4 15.6 15 15 15
y11 (2+) 5.7 4.5 7.1 12.7 7.4 6.8 13.9 8.7 9.8 15 15 15
b2 (1+) 9.2 5.7 6.8 16.8 6.9 7.5 19.2 8.9 10.1 15 15 15
b3 (1+) 10.4 8 5.7 11.8 11.2 9.7 15.7 13.8 11.3 15 15 15
sum 5.3 3 3 14.9 7.1 5.3 15.8 7.7 6.1 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments