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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Igfals

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Igfals Albs, Als
Sequence Length (AA) Molecular Weight (Da)
603 66960
Protein Name
Insulin-like growth factor-binding protein complex acid labile subunit
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MALRTGSPAL VVLLAFWVAL GPCYLQGTDP GASADAEGPQ CPVTCTCSYD
60 70 80 90 100
DYTDELSVFC SSRNLTQLPD GIPVSTRALW LDGNNLSSIP SAAFQNLSSL
110 120 130 140 150
DFLNLQGSWL RSLEPQALLG LQNLYHLHLE RNLLRSLAAG LFRHTPSLAS
160 170 180 190 200
LSLGNNLLGR LEEGLFRGLS HLWDLNLGWN SLVVLPDTVF QGLGNLHELV
210 220 230 240 250
LAGNKLTYLQ PALLCGLGEL RELDLSRNAL RSVKANVFIH LPRLQKLYLD
260 270 280 290 300
RNLITAVAPR AFLGMKALRW LDLSHNRVAG LLEDTFPGLL GLHVLRLAHN
310 320 330 340 350
AITSLRPRTF KDLHFLEELQ LGHNRIRQLG EKTFEGLGQL EVLTLNDNQI
360 370 380 390 400
HEVKVGAFFG LFNVAVMNLS GNCLRSLPEH VFQGLGRLHS LHLEHSCLGR
410 420 430 440 450
IRLHTFAGLS GLRRLFLRDN SISSIEEQSL AGLSELLELD LTANQLTHLP
460 470 480 490 500
RQLFQGLGQL EYLLLSNNQL TMLSEDVLGP LQRAFWLDLS HNRLETPAEG
510 520 530 540 550
LFSSLGRLRY LNLRNNSLQT FVPQPGLERL WLDANPWDCS CPLKALRDFA
560 570 580 590 600
LQNPGVVPRF VQTVCEGDDC QPVYTYNNIT CAGPANVSGL DLRDISETLF
603
VHC

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-5574 Collapse assay details

Data source: Panorama

Official Gene Symbol
Igfals
Peptide Sequence
DFALQNPGVVPR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
548
Peptide End
559
CPTAC ID
non-CPTAC-5574
Peptide Molecular Mass
1,311.6935
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 9.8 6.6 9.6 9.6 10.2 9.7 13.7 12.1 13.6 15 15 15
b2 (1+) 17 9.3 8.9 13.7 8.4 8.4 21.8 12.5 12.2 15 15 15
y2 (1+) 10.1 6.4 5.3 13.2 11.1 7.8 16.6 12.8 9.4 15 15 15
y3 (1+) 10 8.3 7.7 12.8 7.6 8.5 16.2 11.3 11.5 15 15 15
y6 (1+) 7.6 6.9 6.7 13 9.5 5.3 15.1 11.7 8.5 15 15 15
sum 4 1.5 2.5 7 6.3 3.7 8.1 6.5 4.5 15 15 15

Additional Resources and Comments

Resources
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View Peptide in Panorama
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Comments

Assay Details for non-CPTAC-2678 Collapse assay details

Data source: Panorama

Official Gene Symbol
IGFALS
Peptide Sequence
SLALGTFAHTPALASLGLSNNR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
136
Peptide End
157
CPTAC ID
non-CPTAC-2678
Peptide Molecular Mass
2,210.1808
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 5.4 4.7 16.9 19.1 7.8 23.7 19.8 9.1 29.1 15 15 15
b2 (1+) 5.8 4.2 10 18.7 7.3 13.2 19.6 8.4 16.6 15 15 15
b14 (2+) 9.2 5.2 14 18.3 8 19.6 20.5 9.5 24.1 15 15 15
b6 (2+) 7 4.3 12.1 18.7 7.1 15.3 20 8.3 19.5 15 15 15
b17 (2+) 5.7 4.4 16.8 20.1 8.6 22.8 20.9 9.7 28.3 15 15 15
sum 5.7 4.3 13.7 18.9 7.4 18.3 19.7 8.6 22.9 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments