CD5L, CPTAC-1092 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CD5L	API6

Sequence Length (AA)	Molecular Weight (Da)
347	38088

Protein Name
CD5 antigen-like

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MALLFSLILA	ICTRPGFLAS	PSGVRLVGGL	HRCEGRVEVE	QKGQWGTVCD
60	70	80	90	100
DGWDIKDVAV	LCRELGCGAA	SGTPSGILYE	PPAEKEQKVL	IQSVSCTGTE
110	120	130	140	150
DTLAQCEQEE	VYDCSHDEDA	GASCENPESS	FSPVPEGVRL	ADGPGHCKGR
160	170	180	190	200
VEVKHQNQWY	TVCQTGWSLR	AAKVVCRQLG	CGRAVLTQKR	CNKHAYGRKP
210	220	230	240	250
IWLSQMSCSG	REATLQDCPS	GPWGKNTCNH	DEDTWVECED	PFDLRLVGGD
260	270	280	290	300
NLCSGRLEVL	HKGVWGSVCD	DNWGEKEDQV	VCKQLGCGKS	LSPSFRDRKC
310	320	330	340	347
YGPGVGRIWL	DNVRCSGEEQ	SLEQCQHRFW	GFHDCTHQED	VAVICSG

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-5586 Collapse assay details

Data source: Panorama

Official Gene Symbol: Cd5l
Peptide Sequence: IYGPGAGR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 302
Peptide End: 309

CPTAC ID: non-CPTAC-5586
Peptide Molecular Mass: 789.4133
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

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Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >80%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b2 (1+)	5.6	3.4	6	9.5	6.5	8.1	11	7.3	10.1	15	15	15
y4 (1+)	14.3	6.4	7.8	12.1	9.3	6.9	18.7	11.3	10.4	15	15	15
y5 (1+)	8.2	5.3	6.4	11.5	8.3	6.4	14.1	9.8	9.1	15	15	15
y6 (1+)	6.2	3.3	4.5	6.6	6.3	6.8	9.1	7.1	8.2	15	15	15
y7 (1+)	4.1	5.7	4.4	6.7	7.9	5.4	7.9	9.7	7	15	15	15
sum	3.4	2.3	3.7	5.2	6.1	5.4	6.2	6.5	6.5	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-1092 Collapse assay details

Data source: Panorama

Official Gene Symbol: CD5L
Peptide Sequence: LVGGLHR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 26
Peptide End: 32

CPTAC ID: non-CPTAC-1092
Peptide Molecular Mass: 750.4501
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers