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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
CA1 N/A
Sequence Length (AA) Molecular Weight (Da)
261 28870
Protein Name
Carbonic anhydrase 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MASPDWGYDD KNGPEQWSKL YPIANGNNQS PVDIKTSETK HDTSLKPISV
60 70 80 90 100
SYNPATAKEI INVGHSFHVN FEDNDNRSVL KGGPFSDSYR LFQFHFHWGS
110 120 130 140 150
TNEHGSEHTV DGVKYSAELH VAHWNSAKYS SLAEAASKAD GLAVIGVLMK
160 170 180 190 200
VGEANPKLQK VLDALQAIKT KGKRAPFTNF DPSTLLPSSL DFWTYPGSLT
210 220 230 240 250
HPPLYESVTW IICKESISVS SEQLAQFRSL LSNVEGDNAV PMQHNNRPTQ
260 261
PLKGRTVRAS F

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1302 Collapse assay details

Data source: Panorama

Official Gene Symbol
CA1
Peptide Modified Sequence
EIINVGHSFHVNFEDNDN[+1.0]R
Modification Type
Deamidated (N)
Protein - Site of Modification
76
Peptide - Site of Modification
18
Peptide Start
59
Peptide End
77
CPTAC ID
CPTAC-1302
Peptide Molecular Mass
2,256.0196
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tumor tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y14 (2+) 81.6 47.3 60.4 143.7 69.1 67.4 165.3 83.7 90.5 15 15 15
y8 (1+) 104.7 49.1 62.5 121.6 79 77.9 160.5 93 99.9 15 15 15
y9 (1+) 90.8 83.7 75.6 144.5 157.8 80.1 170.7 178.6 110.1 15 15 15
sum 55.9 18.4 33 79 34.3 34.4 96.8 38.9 47.7 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-3566 Collapse assay details

Data source: Panorama

Official Gene Symbol
Ca1
Peptide Sequence
VLDALNSVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
161
Peptide End
169
CPTAC ID
non-CPTAC-3566
Peptide Molecular Mass
957.5495
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b4 (2+) 125.1 118.7 127.6 121.1 131.4 95.5 174.1 177.1 159.4 15 15 15
b2 (1+) 12 7.1 7.8 12.4 8 10 17.3 10.7 12.7 15 15 15
y6 (1+) 54.2 18.1 7.3 55.1 21.7 7.5 77.3 28.3 10.5 15 15 15
y7 (1+) 16 7.9 8.6 14.3 8.8 14.1 21.5 11.8 16.5 15 15 15
y8 (1+) 48.6 8.5 10.4 42 11.7 14.3 64.2 14.5 17.7 15 15 15
sum 13.1 3.4 4.3 12.1 6.8 11.1 17.8 7.6 11.9 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-1091 Collapse assay details

Data source: Panorama

Official Gene Symbol
CA1
Peptide Sequence
VLDALQAIK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
161
Peptide End
169
CPTAC ID
non-CPTAC-1091
Peptide Molecular Mass
969.5859
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 18.7 9.1 6.6 23.6 9.2 8.6 30.1 12.9 10.8 15 15 15
y3 (1+) 11.9 7.6 6.6 16.2 10.6 7.8 20.1 13 10.2 15 15 15
y4 (1+) 15.8 11.3 9 20.5 11.4 7.4 25.9 16.1 11.7 12 15 14
y6 (1+) 19.1 9.7 9.2 21.8 10.6 9.8 29 14.4 13.4 15 15 15
y7 (1+) 8 10.7 3.5 9 9.5 7.9 12 14.3 8.6 15 15 15
sum 7.2 9.4 3.1 7.7 8.4 6.9 10.5 12.6 7.6 15 15 15


Additional Resources and Comments