CA1, CPTAC-1091 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CA1	N/A

Sequence Length (AA)	Molecular Weight (Da)
261	28870

Protein Name
Carbonic anhydrase 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MASPDWGYDD	KNGPEQWSKL	YPIANGNNQS	PVDIKTSETK	HDTSLKPISV
60	70	80	90	100
SYNPATAKEI	INVGHSFHVN	FEDNDNRSVL	KGGPFSDSYR	LFQFHFHWGS
110	120	130	140	150
TNEHGSEHTV	DGVKYSAELH	VAHWNSAKYS	SLAEAASKAD	GLAVIGVLMK
160	170	180	190	200
VGEANPKLQK	VLDALQAIKT	KGKRAPFTNF	DPSTLLPSSL	DFWTYPGSLT
210	220	230	240	250
HPPLYESVTW	IICKESISVS	SEQLAQFRSL	LSNVEGDNAV	PMQHNNRPTQ
260	261
PLKGRTVRAS	F

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1302 Collapse assay details

Data source: Panorama

Official Gene Symbol: CA1
Peptide Modified Sequence: EIINVGHSFHVNFEDNDN[+1.0]R
Modification Type: Deamidated (N)
Protein - Site of Modification: 76
Peptide - Site of Modification: 18
Peptide Start: 59
Peptide End: 77

CPTAC ID: CPTAC-1302
Peptide Molecular Mass: 2,256.0196
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tumor tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Accela 1250 Quaternary Low Pump
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y14 (2+)	81.6	47.3	60.4	143.7	69.1	67.4	165.3	83.7	90.5	15	15	15
y8 (1+)	104.7	49.1	62.5	121.6	79	77.9	160.5	93	99.9	15	15	15
y9 (1+)	90.8	83.7	75.6	144.5	157.8	80.1	170.7	178.6	110.1	15	15	15
sum	55.9	18.4	33	79	34.3	34.4	96.8	38.9	47.7	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-3566 Collapse assay details

Data source: Panorama

Official Gene Symbol: Ca1
Peptide Sequence: VLDALNSVK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 161
Peptide End: 169

CPTAC ID: non-CPTAC-3566
Peptide Molecular Mass: 957.5495
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

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Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.