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Overview Data source: UniProt

Official Gene Symbol Other Aliases
FGB N/A
Sequence Length (AA) Molecular Weight (Da)
491 55928
Protein Name
Fibrinogen beta chain
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MKRMVSWSFH KLKTMKHLLL LLLCVFLVKS QGVNDNEEGF FSARGHRPLD
60 70 80 90 100
KKREEAPSLR PAPPPISGGG YRARPAKAAA TQKKVERKAP DAGGCLHADP
110 120 130 140 150
DLGVLCPTGC QLQEALLQQE RPIRNSVDEL NNNVEAVSQT SSSSFQYMYL
160 170 180 190 200
LKDLWQKRQK QVKDNENVVN EYSSELEKHQ LYIDETVNSN IPTNLRVLRS
210 220 230 240 250
ILENLRSKIQ KLESDVSAQM EYCRTPCTVS CNIPVVSGKE CEEIIRKGGE
260 270 280 290 300
TSEMYLIQPD SSVKPYRVYC DMNTENGGWT VIQNRQDGSV DFGRKWDPYK
310 320 330 340 350
QGFGNVATNT DGKNYCGLPG EYWLGNDKIS QLTRMGPTEL LIEMEDWKGD
360 370 380 390 400
KVKAHYGGFT VQNEANKYQI SVNKYRGTAG NALMDGASQL MGENRTMTIH
410 420 430 440 450
NGMFFSTYDR DNDGWLTSDP RKQCSKEDGG GWWYNRCHAA NPNGRYYWGG
460 470 480 490 491
QYTWDMAKHG TDDGVVWMNW KGSWYSMRKM SMKIRPFFPQ Q

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-1120 Collapse assay details

Data source: Panorama

Official Gene Symbol
FGB
Peptide Sequence
AHYGGFTVQNEANK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
354
Peptide End
367
CPTAC ID
non-CPTAC-1120
Peptide Molecular Mass
1,534.7165
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b7 (1+) 8.3 5.9 5.3 8.6 8.5 6.7 12 10.3 8.5 15 15 15
b2 (1+) 11.2 9.5 9 13.9 10.8 11.4 17.9 14.4 14.5 15 15 15
y6 (2+) 15.3 9.1 6.9 19.6 11.2 7.1 24.9 14.4 9.9 15 15 15
y5 (1+) 8.1 5.4 7.7 9.9 6.8 8.7 12.8 8.7 11.6 15 15 15
y6 (1+) 11.6 8.8 4.7 14.3 8.8 8.9 18.4 12.4 10.1 15 15 15
sum 5.6 2.1 3.1 8.8 5.2 4.9 10.4 5.6 5.8 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3332 Collapse assay details

Data source: Panorama

Official Gene Symbol
Fgb
Peptide Sequence
AHYGGFTVQNEASK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
344
Peptide End
357
CPTAC ID
non-CPTAC-3332
Peptide Molecular Mass
1,507.7056
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b7 (2+) 5.1 5.6 5.5 15.7 6.4 8 16.5 8.5 9.7 15 15 15
b7 (1+) 5.1 3.5 4.4 12.5 6.7 7.1 13.5 7.6 8.4 15 15 15
b2 (1+) 5.2 5.7 4.8 11.3 8.1 8.7 12.4 9.9 9.9 15 15 15
y5 (1+) 5.4 4.6 3.3 12.1 4.9 7.7 13.3 6.7 8.4 15 15 15
y6 (1+) 5.1 5.4 3.6 13.1 7.4 7.5 14.1 9.2 8.3 15 15 15
sum 2.8 2.8 1.9 12.1 5.2 7 12.4 5.9 7.3 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-2665 Collapse assay details

Data source: Panorama

Official Gene Symbol
FGB
Peptide Sequence
HQLYIDETVNSNIPTNLR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
179
Peptide End
196
CPTAC ID
non-CPTAC-2665
Peptide Molecular Mass
2,126.0756
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 14.1 5.9 7.4 19.3 10.8 7.7 23.9 12.3 10.7 15 15 15
y5 (1+) 7.1 7.3 5.6 15.1 10.9 8.1 16.7 13.1 9.8 15 15 15
b13 (2+) 10.9 4.3 4.3 16.3 8.5 5 19.6 9.5 6.6 15 15 15
b12 (2+) 13.1 5 4.6 13.9 13.9 8.3 19.1 14.8 9.5 15 15 15
y5 (2+) 19.8 7.9 6.1 21.9 5.3 7.5 29.5 9.5 9.7 15 15 15
sum 8 3.6 3.9 14.4 8.1 5.6 16.5 8.9 6.8 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-1119 Collapse assay details

Data source: Panorama

Official Gene Symbol
FGB
Peptide Sequence
QGFGNVATNTDGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
301
Peptide End
313
CPTAC ID
non-CPTAC-1119
Peptide Molecular Mass
1,307.6106
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y2 (1+) 6.4 4.8 4.2 11.9 8 4 13.5 9.3 5.8 15 15 15
y5 (1+) 8 4.2 3.5 9.7 6.6 3.6 12.6 7.8 5 15 15 15
y6 (1+) 6.1 3.8 2.8 9.7 7 3.2 11.5 8 4.3 15 15 15
y7 (1+) 4.2 4.4 2.9 12.3 7.5 6.2 13 8.7 6.8 15 15 15
y8 (1+) 6.4 2.1 4.7 11 6.6 5.6 12.7 6.9 7.3 15 15 15
sum 3.1 2.8 2 10.2 6.6 3.7 10.7 7.2 4.2 15 15 15

Additional Resources and Comments