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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

FGA

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
FGA N/A
Sequence Length (AA) Molecular Weight (Da)
866 94973
Protein Name
Fibrinogen alpha chain
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MFSMRIVCLV LSVVGTAWTA DSGEGDFLAE GGGVRGPRVV ERHQSACKDS
60 70 80 90 100
DWPFCSDEDW NYKCPSGCRM KGLIDEVNQD FTNRINKLKN SLFEYQKNNK
110 120 130 140 150
DSHSLTTNIM EILRGDFSSA NNRDNTYNRV SEDLRSRIEV LKRKVIEKVQ
160 170 180 190 200
HIQLLQKNVR AQLVDMKRLE VDIDIKIRSC RGSCSRALAR EVDLKDYEDQ
210 220 230 240 250
QKQLEQVIAK DLLPSRDRQH LPLIKMKPVP DLVPGNFKSQ LQKVPPEWKA
260 270 280 290 300
LTDMPQMRME LERPGGNEIT RGGSTSYGTG SETESPRNPS SAGSWNSGSS
310 320 330 340 350
GPGSTGNRNP GSSGTGGTAT WKPGSSGPGS TGSWNSGSSG TGSTGNQNPG
360 370 380 390 400
SPRPGSTGTW NPGSSERGSA GHWTSESSVS GSTGQWHSES GSFRPDSPGS
410 420 430 440 450
GNARPNNPDW GTFEEVSGNV SPGTRREYHT EKLVTSKGDK ELRTGKEKVT
460 470 480 490 500
SGSTTTTRRS CSKTVTKTVI GPDGHKEVTK EVVTSEDGSD CPEAMDLGTL
510 520 530 540 550
SGIGTLDGFR HRHPDEAAFF DTASTGKTFP GFFSPMLGEF VSETESRGSE
560 570 580 590 600
SGIFTNTKES SSHHPGIAEF PSRGKSSSYS KQFTSSTSYN RGDSTFESKS
610 620 630 640 650
YKMADEAGSE ADHEGTHSTK RGHAKSRPVR DCDDVLQTHP SGTQSGIFNI
660 670 680 690 700
KLPGSSKIFS VYCDQETSLG GWLLIQQRMD GSLNFNRTWQ DYKRGFGSLN
710 720 730 740 750
DEGEGEFWLG NDYLHLLTQR GSVLRVELED WAGNEAYAEY HFRVGSEAEG
760 770 780 790 800
YALQVSSYEG TAGDALIEGS VEEGAEYTSH NNMQFSTFDR DADQWEENCA
810 820 830 840 850
EVYGGGWWYN NCQAANLNGI YYPGGSYDPR NNSPYEIENG VVWVSFRGAD
860 866
YSLRAVRMKI RPLVTQ

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3709 Collapse assay details

Data source: Panorama

Official Gene Symbol
Fga
Peptide Sequence
EINLQDYEGHQK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
192
Peptide End
203
CPTAC ID
non-CPTAC-3709
Peptide Molecular Mass
1,472.6896
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (3+) 14.9 4.9 2.3 16 6.8 7.9 21.9 8.4 8.2 15 15 15
y7 (2+) NA NA NA NA NA NA NA NA NA 0 0 0
y8 (2+) 9.8 5.2 5.2 13.3 6 8.3 16.5 7.9 9.8 15 15 15
y9 (2+) 9 3.3 4.1 13.1 3.9 9.2 15.9 5.1 10.1 15 15 15
y10 (2+) 7.9 5.7 5.3 16 13.5 16.8 17.8 14.7 17.6 15 15 15
b7 (2+) NA NA NA NA NA NA NA NA NA 0 0 0
sum 5.9 3.2 2.9 12.1 7.8 10.7 13.5 8.4 11.1 15 15 15

Additional Resources and Comments

Resources
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View Peptide in Panorama
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Comments

Assay Details for non-CPTAC-1117 Collapse assay details

Data source: Panorama

Official Gene Symbol
FGA
Peptide Sequence
ESSSHHPGIAEFPSR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
559
Peptide End
573
CPTAC ID
non-CPTAC-1117
Peptide Molecular Mass
1,636.7594
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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view larger image
view larger image

Response Curves

Data source: Panorama

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view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (2+) NA NA NA NA NA NA NA NA NA 0 0 0
y9 (2+) 9.6 6.4 8 12 8.6 8.5 15.4 10.7 11.7 15 15 15
y13 (2+) 9.2 3.3 10 8.4 8.7 12.5 12.5 9.3 16 15 15 15
y3 (1+) 14.1 5.1 6.4 14.9 9.4 6 20.5 10.7 8.8 15 15 15
y4 (1+) 11.2 7.7 6.4 8.8 9.6 7.1 14.2 12.3 9.6 15 15 15
sum 6.1 4 3.3 7.2 5.6 4.1 9.4 6.9 5.3 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-1118 Collapse assay details

Data source: Panorama

Official Gene Symbol
FGA
Peptide Sequence
GSESGIFTNTK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
548
Peptide End
558
CPTAC ID
non-CPTAC-1118
Peptide Molecular Mass
1,139.5459
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y2 (1+) 9.7 3.7 4.9 12.7 6.7 5 16 7.7 7 15 15 15
y4 (1+) 10.6 4.7 4.1 15 6.3 4.2 18.4 7.9 5.9 15 15 15
y5 (1+) 10.1 4.4 4.7 9.7 9.1 4.7 14 10.1 6.6 15 15 15
y7 (1+) 7.4 2.8 5.4 11.4 6.1 5.4 13.6 6.7 7.6 15 15 15
y8 (1+) 10.8 5.7 4.5 13.3 7.2 6.3 17.1 9.2 7.7 15 15 15
sum 4.4 1.2 2.1 8.2 6.1 2.9 9.3 6.2 3.6 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments