HPX, CPTAC-1131 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
HPX	N/A

Sequence Length (AA)	Molecular Weight (Da)
462	51676

Protein Name
Hemopexin

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MARVLGAPVA	LGLWSLCWSL	AIATPLPPTS	AHGNVAEGET	KPDPDVTERC
60	70	80	90	100
SDGWSFDATT	LDDNGTMLFF	KGEFVWKSHK	WDRELISERW	KNFPSPVDAA
110	120	130	140	150
FRQGHNSVFL	IKGDKVWVYP	PEKKEKGYPK	LLQDEFPGIP	SPLDAAVECH
160	170	180	190	200
RGECQAEGVL	FFQGDREWFW	DLATGTMKER	SWPAVGNCSS	ALRWLGRYYC
210	220	230	240	250
FQGNQFLRFD	PVRGEVPPRY	PRDVRDYFMP	CPGRGHGHRN	GTGHGNSTHH
260	270	280	290	300
GPEYMRCSPH	LVLSALTSDN	HGATYAFSGT	HYWRLDTSRD	GWHSWPIAHQ
310	320	330	340	350
WPQGPSAVDA	AFSWEEKLYL	VQGTQVYVFL	TKGGYTLVSG	YPKRLEKEVG
360	370	380	390	400
TPHGIILDSV	DAAFICPGSS	RLHIMAGRRL	WWLDLKSGAQ	ATWTELPWPH
410	420	430	440	450
EKVDGALCME	KSLGPNSCSA	NGPGLYLIHG	PNLYCYSDVE	KLNAAKALPQ
460	462
PQNVTSLLGC	TH

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-5608 Collapse assay details

Data source: Panorama

Official Gene Symbol: Hpx
Peptide Sequence: GPDSVFLIK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 103
Peptide End: 111

CPTAC ID: non-CPTAC-5608
Peptide Molecular Mass: 974.5437
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >80%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b3 (1+)	6.4	3.9	2.2	9.4	6.6	2.6	11.4	7.7	3.4	15	15	15
y8 (2+)	6.4	2.6	2.8	6.9	8	4.5	9.4	8.4	5.3	15	15	15
y4 (1+)	6	4.3	3.5	7.7	5.3	4.2	9.8	6.8	5.5	15	15	15
y6 (1+)	4.7	4.5	4.4	7.5	7.2	3.8	8.9	8.5	5.8	15	15	15
y7 (1+)	6.2	4.7	4	7.6	5.7	5.7	9.8	7.4	7	15	15	15
sum	2.7	2	1.5	5.5	5.2	3.3	6.1	5.6	3.6	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-1131 Collapse assay details

Data source: Panorama

Official Gene Symbol: HPX
Peptide Sequence: NFPSPVDAAFR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 92
Peptide End: 102

CPTAC ID: non-CPTAC-1131
Peptide Molecular Mass: 1,219.5986
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers