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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Hpx Hpxn
Sequence Length (AA) Molecular Weight (Da)
460 51318
Protein Name
Hemopexin
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MARTAVALNI LVLLGLCWSL AVASPLPTAN GRVAEVENGT KPDSDVPEHC
60 70 80 90 100
LDTWSFDAAT MDHNGTMLFF KGEFVWRGHS GTRELISARW KNPITSVDAA
110 120 130 140 150
FRGPDSVFLI KEDKVWVYPP EKKENGYPKL FQEEFPGIPY PPDAAVECHR
160 170 180 190 200
GECQSEGVLF FQGNRKWFWD FATRTQKERS WSTVGNCTAA LRWLERYYCF
210 220 230 240 250
QGNKFLRFNP VTGEVPPRYP LDARDYFVSC PGRGHGRPRN GTAHGNSTHP
260 270 280 290 300
MHSRCSPDPG LTALLSDHRG ATYAFTGSHY WRLDSSRDGW HSWPIAHHWP
310 320 330 340 350
QGPSTVDAAF SWDDKVYLIQ GTQVYVFLTK GGNNLVSGYP KRLEKELGSP
360 370 380 390 400
PGISLETIDA AFSCPGSSRL YVSSGRRLWW LDLKSGAQAT WTEVSWPHEK
410 420 430 440 450
VDGALCLDKS LGPNTCSSNG SSLYFIHGPN LYCYSSIDKL NAAKSLPQPQ
460
KVNSILGCSQ

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-5608 Collapse assay details

Data source: Panorama

Official Gene Symbol
Hpx
Peptide Sequence
GPDSVFLIK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
103
Peptide End
111
CPTAC ID
non-CPTAC-5608
Peptide Molecular Mass
974.5437
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 6.4 3.9 2.2 9.4 6.6 2.6 11.4 7.7 3.4 15 15 15
y8 (2+) 6.4 2.6 2.8 6.9 8 4.5 9.4 8.4 5.3 15 15 15
y4 (1+) 6 4.3 3.5 7.7 5.3 4.2 9.8 6.8 5.5 15 15 15
y6 (1+) 4.7 4.5 4.4 7.5 7.2 3.8 8.9 8.5 5.8 15 15 15
y7 (1+) 6.2 4.7 4 7.6 5.7 5.7 9.8 7.4 7 15 15 15
sum 2.7 2 1.5 5.5 5.2 3.3 6.1 5.6 3.6 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-1131 Collapse assay details

Data source: Panorama

Official Gene Symbol
HPX
Peptide Sequence
NFPSPVDAAFR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
92
Peptide End
102
CPTAC ID
non-CPTAC-1131
Peptide Molecular Mass
1,219.5986
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 4.3 4 5.3 12.5 7.4 6.3 13.2 8.4 8.2 15 15 15
y7 (2+) 8.7 3.4 6.4 11.2 7.3 6.2 14.2 8.1 8.9 15 15 15
y9 (2+) 3.8 8.5 5 8.6 6.8 5.3 9.4 10.9 7.3 15 15 15
y9 (1+) 7.1 4.9 4.1 13.8 8.5 6.7 15.5 9.8 7.9 15 15 15
sum 2.8 4.8 3.5 9.4 5.9 4.1 9.8 7.6 5.4 15 15 15


Additional Resources and Comments