Gp1ba, CPTAC-5607 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Gp1ba	N/A

Sequence Length (AA)	Molecular Weight (Da)
734	80055

Protein Name
Platelet glycoprotein Ib alpha chain

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MALLILLFLL	PSPLHSQHTC	SISKVTSLLE	VNCENKKLTA	LPADLPADTG
60	70	80	90	100
ILHLGENQLG	TFSTASLVHF	THLTYLYLDR	CELTSLQTNG	KLIKLENLDL
110	120	130	140	150
SHNNLKSLPS	LGWALPALTT	LDVSFNKLGS	LSPGVLDGLS	QLQELYLQNN
160	170	180	190	200
DLKSLPPGLL	LPTTKLKKLN	LANNKLRELP	SGLLDGLEDL	DTLYLQRNWL
210	220	230	240	250
RTIPKGFFGT	LLLPFVFLHA	NSWYCDCEIL	YFRHWLQENA	NNVYLWKQGV
260	270	280	290	300
DVKDTTPNVA	SVRCANLDNA	PVYSYPGKGC	PTSSGDTDYD	DYDDIPDVPA
310	320	330	340	350
TRTEVKFSTN	TKVHTTHWSL	LAAAPSTSQD	SQMISLPPTH	KPTKKQSTFI
360	370	380	390	400
HTQSPGFTTL	PETMESNPTF	YSLKLNTVLI	PSPTTLEPTS	TQATPEPNIQ
410	420	430	440	450
PMLTTSTLTT	PEHSTTPVPT	TTILTTPEHS	TIPVPTTAIL	TTPKPSTIPV
460	470	480	490	500
PTTATLTTLE	PSTTPVPTTA	TLTTPEPSTT	LVPTTATLTT	PEHSTTPVPT
510	520	530	540	550
TATLTTPEHS	TTPVPTTATL	TTPEPSTTLT	NLVSTISPVL	TTTLTTPEST
560	570	580	590	600
PIETILEQFF	TTELTLLPTL	ESTTTIIPEQ	NSFLNLPEVA	LVSSDTSESS
610	620	630	640	650
PFLNSDFCCF	LPLGFYVLGL	LWLLFASVVL	ILLLTWTWHV	TPHSLDMEQS
660	670	680	690	700
AALATSTHTT	SLEVQRARQV	TMPRAWLLFL	QGSLPTFRSS	LFLWVRPNGR
710	720	730	734
VGPLVAGRRP	SALSQGRGQD	LLGTVGIRYS	GHSL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-5607 Collapse assay details

Data source: Panorama

Official Gene Symbol: Gp1ba
Peptide Sequence: LENLDLSHNNLK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 95
Peptide End: 106

CPTAC ID: non-CPTAC-5607
Peptide Molecular Mass: 1,408.7310
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >80%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b2 (1+)	4.9	2.6	1.8	8.1	5.8	2.8	9.5	6.4	3.3	15	15	15
y9 (2+)	7.7	2.5	4.8	9.6	8.5	5	12.3	8.9	6.9	15	15	15
y10 (2+)	2.7	2.1	1.7	6.9	5.9	2.7	7.4	6.3	3.2	15	15	15
y11 (2+)	3.4	2.5	2	8.3	5.7	2.8	9	6.2	3.4	15	15	15
y8 (1+)	5	4.5	1.6	6.6	7.6	3.8	8.3	8.8	4.1	15	15	15
sum	1.6	1.1	0.9	6.4	5.6	2.3	6.6	5.7	2.5	15	15	15

Gp1ba

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-5607 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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Gp1ba

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-5607 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments