LRG1, CPTAC-1139 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
LRG1	LRG

Sequence Length (AA)	Molecular Weight (Da)
347	38178

Protein Name
Leucine-rich alpha-2-glycoprotein

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSSWSRQRPK	SPGGIQPHVS	RTLFLLLLLA	ASAWGVTLSP	KDCQVFRSDH
60	70	80	90	100
GSSISCQPPA	EIPGYLPADT	VHLAVEFFNL	THLPANLLQG	ASKLQELHLS
110	120	130	140	150
SNGLESLSPE	FLRPVPQLRV	LDLTRNALTG	LPPGLFQASA	TLDTLVLKEN
160	170	180	190	200
QLEVLEVSWL	HGLKALGHLD	LSGNRLRKLP	PGLLANFTLL	RTLDLGENQL
210	220	230	240	250
ETLPPDLLRG	PLQLERLHLE	GNKLQVLGKD	LLLPQPDLRY	LFLNGNKLAR
260	270	280	290	300
VAAGAFQGLR	QLDMLDLSNN	SLASVPEGLW	ASLGQPNWDM	RDGFDISGNP
310	320	330	340	347
WICDQNLSDL	YRWLQAQKDK	MFSQNDTRCA	GPEAVKGQTL	LAVAKSQ

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-678 Collapse assay details

Data source: Panorama

Official Gene Symbol: LRG1
Peptide Modified Sequence: LPPGLLAN[+1.0]FTLLR
Modification Type: Deamidated (NQ)
Protein - Site of Modification: 186
Peptide - Site of Modification: 8
Peptide Start: 179
Peptide End: 191

CPTAC ID: CPTAC-678
Peptide Molecular Mass: 1,424.8391
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: N/A
Matrix: serum
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Q-Exactive (ThermoFisher)
Internal Standard: synthetic peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Dionex UltiMate 3000 RSLCnano LC (ThermoFisher)
Column Packing: Acclaim PepMap100 C18, 5um (Trap); Acclaim PepMap RSLC C18, 2um (Analytical)
Column Dimensions: 300um x 5mm (Trap); 75um x 25cm (Analytical)
Flow Rate: 0.5 uL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y11 (1+)	1.7	1.8	2	6.8	2.1	2	7	2.8	2.8	15	15	15
y8 (1+)	3.6	1.4	2	8	1.9	1.8	8.8	2.4	2.7	15	15	15
y12 (2+)	2.5	1.9	2.3	6.9	2.3	2	7.3	3	3	15	15	15
sum	1.9	1.6	1.9	6.8	2.1	1.7	7.1	2.6	2.5	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-1139 Collapse assay details

Data source: Panorama

Official Gene Symbol: LRG1
Peptide Sequence: DLLLPQPDLR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 230
Peptide End: 239

CPTAC ID: non-CPTAC-1139
Peptide Molecular Mass: 1,178.6659
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers