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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

LBP

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
LBP N/A
Sequence Length (AA) Molecular Weight (Da)
481 53384
Protein Name
Lipopolysaccharide-binding protein
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGALARALPS ILLALLLTST PEALGANPGL VARITDKGLQ YAAQEGLLAL
60 70 80 90 100
QSELLRITLP DFTGDLRIPH VGRGRYEFHS LNIHSCELLH SALRPVPGQG
110 120 130 140 150
LSLSISDSSI RVQGRWKVRK SFFKLQGSFD VSVKGISISV NLLLGSESSG
160 170 180 190 200
RPTVTASSCS SDIADVEVDM SGDLGWLLNL FHNQIESKFQ KVLESRICEM
210 220 230 240 250
IQKSVSSDLQ PYLQTLPVTT EIDSFADIDY SLVEAPRATA QMLEVMFKGE
260 270 280 290 300
IFHRNHRSPV TLLAAVMSLP EEHNKMVYFA ISDYVFNTAS LVYHEEGYLN
310 320 330 340 350
FSITDDMIPP DSNIRLTTKS FRPFVPRLAR LYPNMNLELQ GSVPSAPLLN
360 370 380 390 400
FSPGNLSVDP YMEIDAFVLL PSSSKEPVFR LSVATNVSAT LTFNTSKITG
410 420 430 440 450
FLKPGKVKVE LKESKVGLFN AELLEALLNY YILNTFYPKF NDKLAEGFPL
460 470 480 481
PLLKRVQLYD LGLQIHKDFL FLGANVQYMR V

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3836 Collapse assay details

Data source: Panorama

Official Gene Symbol
Lbp
Peptide Sequence
HIQLHDLDFQIR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
455
Peptide End
466
CPTAC ID
non-CPTAC-3836
Peptide Molecular Mass
1,533.8052
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b6 (2+) 8.8 7.2 10.8 11.1 6.8 15.8 14.2 9.9 19.1 15 15 15
b2 (1+) 11.9 5.5 21.9 21.2 23.8 71.5 24.3 24.4 74.8 15 15 15
y4 (1+) 16.5 12.8 15.5 17.3 10.4 15.9 23.9 16.5 22.2 15 15 15
y5 (1+) 15.8 8 13.3 9.9 8.7 11.1 18.6 11.8 17.3 15 15 15
y7 (1+) 8.1 6.5 7.2 9.3 6.8 11.6 12.3 9.4 13.7 15 15 15
sum 4.7 3.7 5.7 5.2 4.4 18.9 7 5.7 19.7 15 15 15

Additional Resources and Comments

Resources
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Comments

Assay Details for non-CPTAC-1140 Collapse assay details

Data source: Panorama

Official Gene Symbol
LBP
Peptide Sequence
ITLPDFTGDLR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
57
Peptide End
67
CPTAC ID
non-CPTAC-1140
Peptide Molecular Mass
1,246.6558
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 11 6.1 3.7 11.8 6 7.2 16.1 8.6 8.1 15 15 15
y9 (2+) 7.6 7.9 5.4 8.9 9.8 7.3 11.7 12.6 9.1 15 15 15
y2 (1+) 11 6.7 5.2 12.6 9.5 6.3 16.7 11.6 8.2 15 15 14
y6 (1+) 11.7 5.2 6.6 10.5 10.4 9.6 15.7 11.6 11.6 15 15 14
y8 (1+) 8.8 8 4.7 12 10.3 9 14.9 13 10.2 15 15 14
sum 7.3 4.6 5.8 9.3 7.5 6.1 11.8 8.8 8.4 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-3555 Collapse assay details

Data source: Panorama

Official Gene Symbol
Lbp
Peptide Sequence
LLPGQGLSLAISDSSIGVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
95
Peptide End
113
CPTAC ID
non-CPTAC-3555
Peptide Molecular Mass
1,882.0524
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 5.6 4 6 15.6 4.1 9.9 16.6 5.7 11.6 15 15 15
y17 (3+) 6.3 2.9 6.1 15.4 3.4 9.2 16.6 4.5 11 15 15 15
y17 (2+) 5.2 5.6 5.5 17.7 9.3 7.6 18.4 10.9 9.4 15 15 15
y6 (1+) 7.8 6.4 6 14.6 8.1 9.5 16.6 10.3 11.2 15 15 15
y8 (1+) 9 5.5 4 14.1 3.8 5.5 16.7 6.7 6.8 15 15 15
sum 3.6 1.8 3.4 14.7 3.1 5.8 15.1 3.6 6.7 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments