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Overview Data source: UniProt

Official Gene Symbol Other Aliases
SERPINF1 PEDF
Sequence Length (AA) Molecular Weight (Da)
418 46312
Protein Name
Pigment epithelium-derived factor
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MQALVLLLCI GALLGHSSCQ NPASPPEEGS PDPDSTGALV EEEDPFFKVP
60 70 80 90 100
VNKLAAAVSN FGYDLYRVRS STSPTTNVLL SPLSVATALS ALSLGAEQRT
110 120 130 140 150
ESIIHRALYY DLISSPDIHG TYKELLDTVT APQKNLKSAS RIVFEKKLRI
160 170 180 190 200
KSSFVAPLEK SYGTRPRVLT GNPRLDLQEI NNWVQAQMKG KLARSTKEIP
210 220 230 240 250
DEISILLLGV AHFKGQWVTK FDSRKTSLED FYLDEERTVR VPMMSDPKAV
260 270 280 290 300
LRYGLDSDLS CKIAQLPLTG SMSIIFFLPL KVTQNLTLIE ESLTSEFIHD
310 320 330 340 350
IDRELKTVQA VLTVPKLKLS YEGEVTKSLQ EMKLQSLFDS PDFSKITGKP
360 370 380 390 400
IKLTQVEHRA GFEWNEDGAG TTPSPGLQPA HLTFPLDYHL NQPFIFVLRD
410 418
TDTGALLFIG KILDPRGP

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3389 Collapse assay details

Data source: Panorama

Official Gene Symbol
Serpinf1
Peptide Sequence
LAAAVSNFGYDLYR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
53
Peptide End
66
CPTAC ID
non-CPTAC-3389
Peptide Molecular Mass
1,558.7780
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 6.6 5.5 7.5 17 7.4 9.2 18.2 9.2 11.9 15 15 15
y9 (1+) 5.9 4.5 5.9 15.5 5.7 6.3 16.6 7.3 8.6 15 15 15
b5 (1+) 9.5 6 6.3 12.6 7.4 7.2 15.8 9.5 9.6 15 15 15
b4 (1+) 3.7 3.1 3.3 14.3 5.6 4.3 14.8 6.4 5.4 15 15 15
y3 (1+) 13.3 9.3 7.7 20.3 12.4 7.2 24.3 15.5 10.5 15 15 15
sum 3.8 2.5 3.1 14 4.1 3.3 14.5 4.8 4.5 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-1146 Collapse assay details

Data source: Panorama

Official Gene Symbol
SERPINF1
Peptide Sequence
LQSLFDSPDFSK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
334
Peptide End
345
CPTAC ID
non-CPTAC-1146
Peptide Molecular Mass
1,382.6718
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y2 (1+) 5.8 5 8.8 14.1 6.7 8.3 15.2 8.4 12.1 15 15 15
y5 (1+) 11.3 6.7 6.5 22.7 6.1 7.7 25.4 9.1 10.1 15 15 15
y7 (1+) 7.6 5.9 5.2 10.1 6.2 6.6 12.6 8.6 8.4 15 15 15
y8 (1+) 8.7 4.8 6.7 18.3 7.5 5.4 20.3 8.9 8.6 15 15 15
y10 (1+) 7 8.6 3.4 15.1 7.9 5.7 16.6 11.7 6.6 15 15 15
sum 5.3 5.3 3.8 14.8 5.6 4.4 15.7 7.7 5.8 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3390 Collapse assay details

Data source: Panorama

Official Gene Symbol
Serpinf1
Peptide Sequence
LTFPLDYHLNQPFLFVLR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
381
Peptide End
398
CPTAC ID
non-CPTAC-3390
Peptide Molecular Mass
2,232.2095
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 3.5 2.7 3 14 11.3 19.6 14.4 11.6 19.8 15 15 15
y16 (3+) 3.8 2.8 1.9 12.8 8.6 15.5 13.4 9 15.6 15 15 15
y15 (2+) 4.2 2 2.1 13.9 10.3 21 14.5 10.5 21.1 15 15 15
y16 (2+) 1.1 3.2 2.2 12.9 12.5 16.6 12.9 12.9 16.7 15 15 15
y7 (1+) 4.5 4.1 3 12.1 7.8 16 12.9 8.8 16.3 15 15 15
sum 2.7 1.3 1.5 13.3 10.5 18.8 13.6 10.6 18.9 15 15 15

Additional Resources and Comments