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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

PLTP

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
PLTP N/A
Sequence Length (AA) Molecular Weight (Da)
493 54739
Protein Name
Phospholipid transfer protein
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MALFGALFLA LLAGAHAEFP GCKIRVTSKA LELVKQEGLR FLEQELETIT
60 70 80 90 100
IPDLRGKEGH FYYNISEVKV TELQLTSSEL DFQPQQELML QITNASLGLR
110 120 130 140 150
FRRQLLYWFF YDGGYINASA EGVSIRTGLE LSRDPAGRMK VSNVSCQASV
160 170 180 190 200
SRMHAAFGGT FKKVYDFLST FITSGMRFLL NQQICPVLYH AGTVLLNSLL
210 220 230 240 250
DTVPVRSSVD ELVGIDYSLM KDPVASTSNL DMDFRGAFFP LTERNWSLPN
260 270 280 290 300
RAVEPQLQEE ERMVYVAFSE FFFDSAMESY FRAGALQLLL VGDKVPHDLD
310 320 330 340 350
MLLRATYFGS IVLLSPAVID SPLKLELRVL APPRCTIKPS GTTISVTASV
360 370 380 390 400
TIALVPPDQP EVQLSSMTMD ARLSAKMALR GKALRTQLDL RRFRIYSNHS
410 420 430 440 450
ALESLALIPL QAPLKTMLQI GVMPMLNERT WRGVQIPLPE GINFVHEVVT
460 470 480 490 493
NHAGFLTIGA DLHFAKGLRE VIEKNRPADV RASTAPTPST AAV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1321 Collapse assay details

Data source: Panorama

Official Gene Symbol
PLTP
Peptide Modified Sequence
N[+1.0]WSLPNR
Modification Type
Deamidated (N)
Protein - Site of Modification
245
Peptide - Site of Modification
1
Peptide Start
245
Peptide End
251
CPTAC ID
CPTAC-1321
Peptide Molecular Mass
886.4297
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tumor tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 48.3 14.3 13.1 44.2 13 13.5 65.5 19.3 18.8 15 15 15
y4 (1+) 122 53.4 32.2 136.2 54.5 31 182.9 76.3 44.7 15 15 15
y3 (1+) 96.9 24.4 31 81.1 35.2 42.3 126.4 42.8 52.4 15 15 15
sum 56 17.2 13.8 47.9 16.4 11.7 73.7 23.8 18.1 15 15 15

Additional Resources and Comments

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Assay Details for non-CPTAC-5614 Collapse assay details

Data source: Panorama

Official Gene Symbol
Pltp
Peptide Sequence
AVEPQLEDDER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
252
Peptide End
262
CPTAC ID
non-CPTAC-5614
Peptide Molecular Mass
1,299.5943
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 19 7.1 9 17.7 9 10.3 26 11.5 13.7 15 15 15
y8 (2+) 14.4 9.1 5.5 17.5 9.9 6.9 22.7 13.4 8.8 15 15 15
y9 (2+) 27.4 12 7.2 28.5 13 7.5 39.5 17.7 10.4 15 15 15
y2 (1+) 14.5 7.4 8.3 24.5 10.8 11.4 28.5 13.1 14.1 15 15 15
y8 (1+) 8.9 5.8 4.6 9.1 8.7 4.3 12.7 10.5 6.3 15 15 15
sum 9.1 5.7 3.4 11.9 7.9 4.6 15 9.7 5.7 15 15 15

Additional Resources and Comments

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Assay Details for non-CPTAC-1145 Collapse assay details

Data source: Panorama

Official Gene Symbol
PLTP
Peptide Sequence
AVEPQLQEEER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
252
Peptide End
262
CPTAC ID
non-CPTAC-1145
Peptide Molecular Mass
1,326.6416
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 23.2 8.7 4.6 26.6 13.6 6.5 35.3 16.1 8 14 15 15
b3 (1+) 17.7 12.9 6.5 30.6 12.6 6.1 35.4 18 8.9 15 15 15
y8 (2+) 12.3 8.2 8.4 12.3 8.2 8.5 17.4 11.6 12 15 15 15
y9 (2+) 36.1 10.3 8.9 44 17.8 8 56.9 20.6 12 14 15 15
y6 (1+) 25.4 12.9 10.6 37.4 11.4 10.3 45.2 17.2 14.8 14 15 15
sum 9.8 6.3 5.7 7.5 7.2 5.8 12.3 9.6 8.1 15 15 15

Additional Resources and Comments

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Assay Details for non-CPTAC-5613 Collapse assay details

Data source: Panorama

Official Gene Symbol
Pltp
Peptide Sequence
VTSAALDLVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
26
Peptide End
35
CPTAC ID
non-CPTAC-5613
Peptide Molecular Mass
1,015.5914
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 9.1 4.8 4.6 10 7.4 6.1 13.5 8.8 7.6 15 15 15
y6 (1+) 13.6 4.7 4.7 11.9 7.3 4.8 18.1 8.7 6.7 15 15 15
y7 (1+) 8.9 6.1 4.4 9.6 7.8 5.3 13.1 9.9 6.9 15 15 15
y8 (1+) 6.1 3 4.9 6.8 6.3 5 9.1 7 7 15 15 15
y9 (1+) 9 3.5 4.2 10 7.9 5.1 13.5 8.6 6.6 15 15 15
sum 4.6 2.2 3.4 6.7 6.1 4.4 8.1 6.5 5.6 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments