Overview Data source: UniProt

Official Gene Symbol Other Aliases
THBS1 TSP,TSP1
Sequence Length (AA) Molecular Weight (Da)
1170 129383
Protein Name
Thrombospondin-1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGLAWGLGVL FLMHVCGTNR IPESGGDNSV FDIFELTGAA RKGSGRRLVK
60 70 80 90 100
GPDPSSPAFR IEDANLIPPV PDDKFQDLVD AVRAEKGFLL LASLRQMKKT
110 120 130 140 150
RGTLLALERK DHSGQVFSVV SNGKAGTLDL SLTVQGKQHV VSVEEALLAT
160 170 180 190 200
GQWKSITLFV QEDRAQLYID CEKMENAELD VPIQSVFTRD LASIARLRIA
210 220 230 240 250
KGGVNDNFQG VLQNVRFVFG TTPEDILRNK GCSSSTSVLL TLDNNVVNGS
260 270 280 290 300
SPAIRTNYIG HKTKDLQAIC GISCDELSSM VLELRGLRTI VTTLQDSIRK
310 320 330 340 350
VTEENKELAN ELRRPPLCYH NGVQYRNNEE WTVDSCTECH CQNSVTICKK
360 370 380 390 400
VSCPIMPCSN ATVPDGECCP RCWPSDSADD GWSPWSEWTS CSTSCGNGIQ
410 420 430 440 450
QRGRSCDSLN NRCEGSSVQT RTCHIQECDK RFKQDGGWSH WSPWSSCSVT
460 470 480 490 500
CGDGVITRIR LCNSPSPQMN GKPCEGEARE TKACKKDACP INGGWGPWSP
510 520 530 540 550
WDICSVTCGG GVQKRSRLCN NPTPQFGGKD CVGDVTENQI CNKQDCPIDG
560 570 580 590 600
CLSNPCFAGV KCTSYPDGSW KCGACPPGYS GNGIQCTDVD ECKEVPDACF
610 620 630 640 650
NHNGEHRCEN TDPGYNCLPC PPRFTGSQPF GQGVEHATAN KQVCKPRNPC
660 670 680 690 700
TDGTHDCNKN AKCNYLGHYS DPMYRCECKP GYAGNGIICG EDTDLDGWPN
710 720 730 740 750
ENLVCVANAT YHCKKDNCPN LPNSGQEDYD KDGIGDACDD DDDNDKIPDD
760 770 780 790 800
RDNCPFHYNP AQYDYDRDDV GDRCDNCPYN HNPDQADTDN NGEGDACAAD
810 820 830 840 850
IDGDGILNER DNCQYVYNVD QRDTDMDGVG DQCDNCPLEH NPDQLDSDSD
860 870 880 890 900
RIGDTCDNNQ DIDEDGHQNN LDNCPYVPNA NQADHDKDGK GDACDHDDDN
910 920 930 940 950
DGIPDDKDNC RLVPNPDQKD SDGDGRGDAC KDDFDHDSVP DIDDICPENV
960 970 980 990 1000
DISETDFRRF QMIPLDPKGT SQNDPNWVVR HQGKELVQTV NCDPGLAVGY
1010 1020 1030 1040 1050
DEFNAVDFSG TFFINTERDD DYAGFVFGYQ SSSRFYVVMW KQVTQSYWDT
1060 1070 1080 1090 1100
NPTRAQGYSG LSVKVVNSTT GPGEHLRNAL WHTGNTPGQV RTLWHDPRHI
1110 1120 1130 1140 1150
GWKDFTAYRW RLSHRPKTGF IRVVMYEGKK IMADSGPIYD KTYAGGRLGL
1160 1170
FVFSQEMVFF SDLKYECRDP

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
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Assay Details for non-CPTAC-1162 Collapse assay details

Data source: Panorama

Official Gene Symbol
THBS1
Peptide Sequence
GTLLALER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
102
Peptide End
109
CPTAC ID
non-CPTAC-1162
Peptide Molecular Mass
871.5127
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b4 (1+) 12.7 8.3 7.3 13.9 9.5 6.6 18.8 12.6 9.8 13 15 15
b3 (1+) 22.2 7.3 4.5 21.8 9 5.3 31.1 11.6 7 14 15 15
y4 (1+) 7.7 5.1 5.4 5.9 5.3 6.3 9.7 7.4 8.3 15 15 15
y5 (1+) 9.7 7.2 5.1 9.8 7.9 5.3 13.8 10.7 7.4 15 15 15
sum 4.1 5.4 3.5 4.7 5.6 3.9 6.2 7.8 5.2 15 15 15

Additional Resources and Comments