TFPI, CPTAC-1163 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
TFPI	LACI, TFPI1

Sequence Length (AA)	Molecular Weight (Da)
304	35015

Protein Name
Tissue factor pathway inhibitor

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MIYTMKKVHA	LWASVCLLLN	LAPAPLNADS	EEDEEHTIIT	DTELPPLKLM
60	70	80	90	100
HSFCAFKADD	GPCKAIMKRF	FFNIFTRQCE	EFIYGGCEGN	QNRFESLEEC
110	120	130	140	150
KKMCTRDNAN	RIIKTTLQQE	KPDFCFLEED	PGICRGYITR	YFYNNQTKQC
160	170	180	190	200
ERFKYGGCLG	NMNNFETLEE	CKNICEDGPN	GFQVDNYGTQ	LNAVNNSLTP
210	220	230	240	250
QSTKVPSLFE	FHGPSWCLTP	ADRGLCRANE	NRFYYNSVIG	KCRPFKYSGC
260	270	280	290	300
GGNENNFTSK	QECLRACKKG	FIQRISKGGL	IKTKRKRKKQ	RVKIAYEEIF
304
VKNM

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-1163 Collapse assay details

Data source: Panorama

Official Gene Symbol: TFPI
Peptide Sequence: FYYNSVIGK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 233
Peptide End: 241

CPTAC ID: non-CPTAC-1163
Peptide Molecular Mass: 1,089.5495
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 6490 Triple Quad (Agilent)
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b2 (1+)	12.2	10.6	6.2	12.7	10.8	5.9	17.6	15.1	8.6	15	15	15
y3 (1+)	27.8	9.4	6.2	31	12.9	5.2	41.6	16	8.1	15	15	15
y6 (1+)	19.9	7.7	8.5	24.2	8.4	10.4	31.3	11.4	13.4	13	15	15
y7 (1+)	10.2	9	6.1	13.1	9.2	6.1	16.6	12.9	8.6	15	15	15
y8 (1+)	23.5	8.2	9.6	24.9	9.6	9.1	34.2	12.6	13.2	14	15	15
sum	5.8	7.3	4.3	6	7.4	4.3	8.3	10.4	6.1	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-4064 Collapse assay details

Data source: Panorama

Official Gene Symbol: Tfpi
Peptide Sequence: STVNNIVVPQSPK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 200
Peptide End: 212

CPTAC ID: non-CPTAC-4064
Peptide Molecular Mass: 1,381.7565
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers