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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
TFPI LACI, TFPI1
Sequence Length (AA) Molecular Weight (Da)
304 35015
Protein Name
Tissue factor pathway inhibitor
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MIYTMKKVHA LWASVCLLLN LAPAPLNADS EEDEEHTIIT DTELPPLKLM
60 70 80 90 100
HSFCAFKADD GPCKAIMKRF FFNIFTRQCE EFIYGGCEGN QNRFESLEEC
110 120 130 140 150
KKMCTRDNAN RIIKTTLQQE KPDFCFLEED PGICRGYITR YFYNNQTKQC
160 170 180 190 200
ERFKYGGCLG NMNNFETLEE CKNICEDGPN GFQVDNYGTQ LNAVNNSLTP
210 220 230 240 250
QSTKVPSLFE FHGPSWCLTP ADRGLCRANE NRFYYNSVIG KCRPFKYSGC
260 270 280 290 300
GGNENNFTSK QECLRACKKG FIQRISKGGL IKTKRKRKKQ RVKIAYEEIF
304
VKNM

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-1163 Collapse assay details

Data source: Panorama

Official Gene Symbol
TFPI
Peptide Sequence
FYYNSVIGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
233
Peptide End
241
CPTAC ID
non-CPTAC-1163
Peptide Molecular Mass
1,089.5495
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 12.2 10.6 6.2 12.7 10.8 5.9 17.6 15.1 8.6 15 15 15
y3 (1+) 27.8 9.4 6.2 31 12.9 5.2 41.6 16 8.1 15 15 15
y6 (1+) 19.9 7.7 8.5 24.2 8.4 10.4 31.3 11.4 13.4 13 15 15
y7 (1+) 10.2 9 6.1 13.1 9.2 6.1 16.6 12.9 8.6 15 15 15
y8 (1+) 23.5 8.2 9.6 24.9 9.6 9.1 34.2 12.6 13.2 14 15 15
sum 5.8 7.3 4.3 6 7.4 4.3 8.3 10.4 6.1 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-4064 Collapse assay details

Data source: Panorama

Official Gene Symbol
Tfpi
Peptide Sequence
STVNNIVVPQSPK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
200
Peptide End
212
CPTAC ID
non-CPTAC-4064
Peptide Molecular Mass
1,381.7565
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 3.6 5.6 4.9 11.3 6.1 5.4 11.9 8.3 7.3 15 15 15
y6 (1+) 4.7 7.2 4 15 7.2 7.4 15.7 10.2 8.4 15 15 15
y5 (1+) 5.5 4.5 2.8 11.5 5.4 4.6 12.7 7 5.4 15 15 15
y3 (1+) 17.8 7 10.1 18.8 8.3 11.3 25.9 10.9 15.2 15 15 15
y2 (1+) 9.1 6.2 9.7 12.8 8.5 9.3 15.7 10.5 13.4 15 15 15
sum 3 3.8 2 10 4.3 4 10.4 5.7 4.5 15 15 15


Additional Resources and Comments