Tfpi, CPTAC-4064 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Tfpi	N/A

Sequence Length (AA)	Molecular Weight (Da)
306	34987

Protein Name
Tissue factor pathway inhibitor

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MTYKMKKEYA	FWATVCLLLS	LVPEFLNALS	EEADDTDSEL	GSMKPLHTFC
60	70	80	90	100
AMKADDGPCK	AMIRSYFFNM	YTHQCEEFIY	GGCEGNENRF	DTLEECKKTC
110	120	130	140	150
IPGYEKTAVK	AASGAERPDF	CFLEEDPGLC	RGYMKRYLYN	NQTKQCERFV
160	170	180	190	200
YGGCLGNRNN	FETLDECKKI	CENPVHSPSP	VNEVQMSDYV	TDGNTVTDRS
210	220	230	240	250
TVNNIVVPQS	PKVPRRRDYR	GRPWCLQPAD	SGLCKASERR	FYYNSATGKC
260	270	280	290	300
HRFNYTGCGG	NNNNFTTRRR	CLRSCKTGLI	KNKSKGVVKI	QRRKAPFVKV
306
VYESIN

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for non-CPTAC-1163 Collapse assay details

Data source: Panorama

Official Gene Symbol: TFPI
Peptide Sequence: FYYNSVIGK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 233
Peptide End: 241

CPTAC ID: non-CPTAC-1163
Peptide Molecular Mass: 1,089.5495
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 6490 Triple Quad (Agilent)
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b2 (1+)	12.2	10.6	6.2	12.7	10.8	5.9	17.6	15.1	8.6	15	15	15
y3 (1+)	27.8	9.4	6.2	31	12.9	5.2	41.6	16	8.1	15	15	15
y6 (1+)	19.9	7.7	8.5	24.2	8.4	10.4	31.3	11.4	13.4	13	15	15
y7 (1+)	10.2	9	6.1	13.1	9.2	6.1	16.6	12.9	8.6	15	15	15
y8 (1+)	23.5	8.2	9.6	24.9	9.6	9.1	34.2	12.6	13.2	14	15	15
sum	5.8	7.3	4.3	6	7.4	4.3	8.3	10.4	6.1	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-4064 Collapse assay details

Data source: Panorama

Official Gene Symbol: Tfpi
Peptide Sequence: STVNNIVVPQSPK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 200
Peptide End: 212

CPTAC ID: non-CPTAC-4064
Peptide Molecular Mass: 1,381.7565
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers