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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Serpina3k

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Serpina3k Mcm2, Spi2
Sequence Length (AA) Molecular Weight (Da)
418 46880
Protein Name
Serine protease inhibitor A3K
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAFIVAMGMI LMAGICPAVL CFPDGTKEMD IVFHEHQDNG TQDDSLTLAS
60 70 80 90 100
VNTDFAFSLY KKLALKNPDT NIVFSPLSIS AALALVSLGA KGKTMEEILE
110 120 130 140 150
GLKFNLTETP EADIHQGFGN LLQSLSQPED QDQINIGNAM FIEKDLQILA
160 170 180 190 200
EFHEKTRALY QTEAFTADFQ QPTEAKNLIN DYVSNQTQGM IKELISELDE
210 220 230 240 250
RTLMVLVNYI YFKGKWKISF DPQDTFESEF YLDEKRSVKV PMMKMKLLTT
260 270 280 290 300
RHFRDEELSC SVLELKYTGN ASALLILPDQ GRMQQVEASL QPETLRKWRK
310 320 330 340 350
TLFPSQIEEL NLPKFSIASN YRLEEDVLPE MGIKEVFTEQ ADLSGITETK
360 370 380 390 400
KLSVSQVVHK AVLDVAETGT EAAAATGVIG GIRKAILPAV HFNRPFLFVI
410 418
YHTSAQSILF MAKVNNPK

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-4063 Collapse assay details

Data source: Panorama

Official Gene Symbol
Serpina3k
Peptide Sequence
ALYQTEAFTADFQQPTEAK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
158
Peptide End
176
CPTAC ID
non-CPTAC-4063
Peptide Molecular Mass
2,158.0219
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b14 (2+) 7.8 5 3.9 7.9 6.2 5.2 11.1 8 6.5 15 15 15
y2 (1+) 5.3 3 2 7.3 3 3.5 9 4.2 4 15 15 15
y5 (1+) 2.7 2.6 3 6.1 4 5.2 6.7 4.8 6 15 15 15
y6 (1+) 6.9 1.9 2.4 7.7 4.3 3.1 10.3 4.7 3.9 15 15 15
y7 (1+) 9.7 4.1 3.3 11.4 6 3.7 15 7.3 5 15 15 15
sum 4 1.3 1.7 6.3 3.6 3.6 7.5 3.8 4 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments