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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Cd84 Slamf5
Sequence Length (AA) Molecular Weight (Da)
329 37378
Protein Name
SLAM family member 5
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAQRHLWIWF LCLQTWSEAA GKDADPMVMN GILGESVTFL LNIQEPKKID
60 70 80 90 100
NIAWTSQSSV AFIKPGVNKA EVTITQGTYK GRIEIIDQKY DLVIRDLRME
110 120 130 140 150
DAGTYKADIN EENEETITKI YYLHIYRRLK TPKITQSLIS SLNNTCNITL
160 170 180 190 200
TCSVEKEEKD VTYSWSPFGE KSNVLQIVHS PMDQKLTYTC TAQNPVSNSS
210 220 230 240 250
DSVTVQQPCT DTPSFHPRHA VLPGGLAVLF LLILIPMLAF LFRLYKRRRD
260 270 280 290 300
RIVLEADDVS KKTVYAVVSR NAQPTESRIY DEIPQSKMLS CKEDPVTTIY
310 320 329
SSVQLSEKMK ETNMKDRSLP KALGNEIVV

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-4062 Collapse assay details

Data source: Panorama

Official Gene Symbol
Cd84
Peptide Sequence
IEIIDQK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
83
Peptide End
89
CPTAC ID
non-CPTAC-4062
Peptide Molecular Mass
857.4858
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b4 (2+) 118.4 114.3 90.4 108.4 143.7 78.9 160.5 183.6 120 15 15 15
b2 (1+) 3.4 3.1 2.4 12.5 5.1 5.2 13 6 5.7 15 15 15
y4 (1+) 8.9 5.4 3.5 11.3 6.1 4 14.4 8.1 5.3 15 15 15
y5 (1+) 6.7 4.5 3.6 8.8 5.9 5.6 11.1 7.4 6.7 15 15 15
y6 (1+) 9.6 4.4 3.7 14.1 9 5.2 17.1 10 6.4 15 15 15
sum 4 2.3 1.7 9.6 4.4 4.3 10.4 5 4.6 15 15 15


Additional Resources and Comments