C1QC, CPTAC-2652 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
C1QC	C1QG

Sequence Length (AA)	Molecular Weight (Da)
245	25774

Protein Name
Complement C1q subcomponent subunit C

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MDVGPSSLPH	LGLKLLLLLL	LLPLRGQANT	GCYGIPGMPG	LPGAPGKDGY
60	70	80	90	100
DGLPGPKGEP	GIPAIPGIRG	PKGQKGEPGL	PGHPGKNGPM	GPPGMPGVPG
110	120	130	140	150
PMGIPGEPGE	EGRYKQKFQS	VFTVTRQTHQ	PPAPNSLIRF	NAVLTNPQGD
160	170	180	190	200
YDTSTGKFTC	KVPGLYYFVY	HASHTANLCV	LLYRSGVKVV	TFCGHTSKTN
210	220	230	240	245
QVNSGGVLLR	LQVGEEVWLA	VNDYYDMVGI	QGSDSVFSGF	LLFPD

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-2652 Collapse assay details

Data source: Panorama

Official Gene Symbol: C1QC
Peptide Sequence: FNAVLTNPQGDYDTSTGK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 140
Peptide End: 157

CPTAC ID: non-CPTAC-2652
Peptide Molecular Mass: 1,926.8959
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 6490 Triple Quad (Agilent)
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y11 (2+)	11.3	7.9	6.4	12.1	9.2	9.4	16.6	12.1	11.4	15	15	15
b2 (1+)	11.5	5.1	5.7	11.5	11.1	7.7	16.3	12.2	9.6	15	15	15
y9 (1+)	9.5	4.4	4.7	18.1	4.9	11.9	20.4	6.6	12.8	15	15	15
y14 (2+)	10.4	7	3.4	13.9	8.9	4.4	17.4	11.3	5.6	15	15	15
b3 (1+)	7.2	7.8	6.9	13.6	9.1	6.6	15.4	12	9.5	15	15	15
sum	6.4	4.8	3.4	9.5	6.9	4.9	11.5	8.4	6	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-3357 Collapse assay details

Data source: Panorama

Official Gene Symbol: C1qc
Peptide Sequence: FNSVVTNPQGHYNPSTGK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 141
Peptide End: 158

CPTAC ID: non-CPTAC-3357
Peptide Molecular Mass: 1,945.9282
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.