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Overview Data source: UniProt

Official Gene Symbol Other Aliases
IGF1 IBP1
Sequence Length (AA) Molecular Weight (Da)
195 21841
Protein Name
Insulin-like growth factor I
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGKISSLPTQ LFKCCFCDFL KVKMHTMSSS HLFYLALCLL TFTSSATAGP
60 70 80 90 100
ETLCGAELVD ALQFVCGDRG FYFNKPTGYG SSSRRAPQTG IVDECCFRSC
110 120 130 140 150
DLRRLEMYCA PLKPAKSARS VRAQRHTDMP KTQKYQPPST NKNTKSQRRK
160 170 180 190 195
GWPKTHPGGE QKEGTEASLQ IRGKKKEQRR EIGSRNAECR GKKGK

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3716 Collapse assay details

Data source: Panorama

Official Gene Symbol
Igf1
Peptide Sequence
GFYFNKPTGYGSSIR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
70
Peptide End
84
CPTAC ID
non-CPTAC-3716
Peptide Molecular Mass
1,692.8260
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (2+) 18.8 9.1 6.8 22.4 11.7 8 29.2 14.8 10.5 15 15 15
y12 (2+) 14.8 6.6 6.1 14.9 6.2 5.4 21 9.1 8.1 15 15 15
y9 (1+) 9.5 6.7 5.7 10.8 10.6 6 14.4 12.5 8.3 15 15 15
b2 (1+) 9.4 6.6 7.6 19.5 9.2 7.3 21.6 11.3 10.5 15 15 15
y13 (3+) 13.8 5 3.4 15 5.4 5.2 20.4 7.4 6.2 15 15 15
sum 5 2.6 3.3 9.2 5.3 3.8 10.5 5.9 5 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-2677 Collapse assay details

Data source: Panorama

Official Gene Symbol
IGF1
Peptide Sequence
GFYFNKPTGYGSSSR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
70
Peptide End
84
CPTAC ID
non-CPTAC-2677
Peptide Molecular Mass
1,666.7740
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 12.6 8.5 11.5 20.4 9 15.8 24 12.4 19.5 15 15 15
y13 (3+) 6 7.6 10.9 10.1 11 11.2 11.7 13.4 15.6 15 15 15
y12 (2+) 6.9 7.6 11.4 13 10.2 15.5 14.7 12.7 19.2 15 15 15
y13 (2+) 6.6 6 10 10.1 6.4 14.5 12.1 8.8 17.6 15 15 15
y11 (2+) 8.8 6.7 10.7 14.6 7.2 12.8 17 9.8 16.7 15 15 15
sum 3.8 5.4 9.8 10 7.2 12.3 10.7 9 15.7 15 15 15

Additional Resources and Comments