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Overview Data source: UniProt

Official Gene Symbol Other Aliases
MASP1 CRARF, CRARF1, PRSS5
Sequence Length (AA) Molecular Weight (Da)
699 79247
Protein Name
Mannan-binding lectin serine protease 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MRWLLLYYAL CFSLSKASAH TVELNNMFGQ IQSPGYPDSY PSDSEVTWNI
60 70 80 90 100
TVPDGFRIKL YFMHFNLESS YLCEYDYVKV ETEDQVLATF CGRETTDTEQ
110 120 130 140 150
TPGQEVVLSP GSFMSITFRS DFSNEERFTG FDAHYMAVDV DECKEREDEE
160 170 180 190 200
LSCDHYCHNY IGGYYCSCRF GYILHTDNRT CRVECSDNLF TQRTGVITSP
210 220 230 240 250
DFPNPYPKSS ECLYTIELEE GFMVNLQFED IFDIEDHPEV PCPYDYIKIK
260 270 280 290 300
VGPKVLGPFC GEKAPEPIST QSHSVLILFH SDNSGENRGW RLSYRAAGNE
310 320 330 340 350
CPELQPPVHG KIEPSQAKYF FKDQVLVSCD TGYKVLKDNV EMDTFQIECL
360 370 380 390 400
KDGTWSNKIP TCKIVDCRAP GELEHGLITF STRNNLTTYK SEIKYSCQEP
410 420 430 440 450
YYKMLNNNTG IYTCSAQGVW MNKVLGRSLP TCLPVCGLPK FSRKLMARIF
460 470 480 490 500
NGRPAQKGTT PWIAMLSHLN GQPFCGGSLL GSSWIVTAAH CLHQSLDPED
510 520 530 540 550
PTLRDSDLLS PSDFKIILGK HWRLRSDENE QHLGVKHTTL HPQYDPNTFE
560 570 580 590 600
NDVALVELLE SPVLNAFVMP ICLPEGPQQE GAMVIVSGWG KQFLQRFPET
610 620 630 640 650
LMEIEIPIVD HSTCQKAYAP LKKKVTRDMI CAGEKEGGKD ACAGDSGGPM
660 670 680 690 699
VTLNRERGQW YLVGTVSWGD DCGKKDRYGV YSYIHHNKDW IQRVTGVRN

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3723 Collapse assay details

Data source: Panorama

Official Gene Symbol
Masp1
Peptide Sequence
SPEPISTQTHSVQILFR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
269
Peptide End
285
CPTAC ID
non-CPTAC-3723
Peptide Molecular Mass
1,939.0163
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y15 (2+) 12.3 3.5 2.5 12.5 4.6 4.5 17.5 5.8 5.1 15 15 15
y14 (2+) 4.9 2.1 1.5 9.4 3.2 4.1 10.6 3.8 4.4 15 15 15
b3 (1+) 9.4 2.7 1.3 10.8 4.5 3.8 14.3 5.2 4 15 15 15
y14 (3+) 10.3 1.8 2.3 16.6 2.3 3.8 19.5 2.9 4.4 15 15 15
y12 (2+) 12.4 3.6 1.4 16 3.9 3.5 20.2 5.3 3.8 15 15 15
sum 3 1.7 0.8 8.1 3 3.6 8.6 3.4 3.7 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-2682 Collapse assay details

Data source: Panorama

Official Gene Symbol
MASP1
Peptide Sequence
TGVITSPDFPNPYPK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
194
Peptide End
208
CPTAC ID
non-CPTAC-2682
Peptide Molecular Mass
1,631.8195
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y2 (1+) 8.7 6.3 7.3 14.5 8.3 8.6 16.9 10.4 11.3 15 15 15
y12 (2+) 14.9 7.2 14.7 20.4 10.6 17.6 25.3 12.8 22.9 15 15 15
b3 (1+) 7.2 3.1 4.5 8.5 6.5 5.6 11.1 7.2 7.2 15 15 15
b4 (1+) 7.1 4.8 7.9 9.7 7.1 9.8 12 8.6 12.6 15 15 15
y9 (1+) 9.4 5.6 6.8 13.8 7.2 7.3 16.7 9.1 10 15 15 15
sum 4.2 3.1 5.3 10 5.6 6.5 10.8 6.4 8.4 15 15 15

Additional Resources and Comments