Masp1, CPTAC-3723 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Masp1	Crarf, Masp3

Sequence Length (AA)	Molecular Weight (Da)
704	79968

Protein Name
Mannan-binding lectin serine protease 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MRFLSFWRLL	LYHALCLALP	EVSAHTVELN	EMFGQIQSPG	YPDSYPSDSE
60	70	80	90	100
VTWNITVPEG	FRIKLYFMHF	NLESSYLCEY	DYVKVETEDQ	VLATFCGRET
110	120	130	140	150
TDTEQTPGQE	VVLSPGTFMS	VTFRSDFSNE	ERFTGFDAHY	MAVDVDECKE
160	170	180	190	200
REDEELSCDH	YCHNYIGGYY	CSCRFGYILH	TDNRTCRVEC	SGNLFTQRTG
210	220	230	240	250
TITSPDYPNP	YPKSSECSYT	IDLEEGFMVS	LQFEDIFDIE	DHPEVPCPYD
260	270	280	290	300
YIKIKAGSKV	WGPFCGEKSP	EPISTQTHSV	QILFRSDNSG	ENRGWRLSYR
310	320	330	340	350
AAGNECPKLQ	PPVYGKIEPS	QAVYSFKDQV	LVSCDTGYKV	LKDNEVMDTF
360	370	380	390	400
QIECLKDGAW	SNKIPTCKIV	DCGAPAGLKH	GLVTFSTRNN	LTTYKSEIRY
410	420	430	440	450
SCQQPYYKML	HNTTGVYTCS	AHGTWTNEVL	KRSLPTCLPV	CGVPKFSRKQ
460	470	480	490	500
ISRIFNGRPA	QKGTMPWIAM	LSHLNGQPFC	GGSLLGSNWV	LTAAHCLHQS
510	520	530	540	550
LDPEEPTLHS	SYLLSPSDFK	IIMGKHWRRR	SDEDEQHLHV	KRTTLHPLYN
560	570	580	590	600
PSTFENDLGL	VELSESPRLN	DFVMPVCLPE	QPSTEGTMVI	VSGWGKQFLQ
610	620	630	640	650
RFPENLMEIE	IPIVNSDTCQ	EAYTPLKKKV	TKDMICAGEK	EGGKDACAGD
660	670	680	690	700
SGGPMVTKDA	ERDQWYLVGV	VSWGEDCGKK	DRYGVYSYIY	PNKDWIQRIT
704
GVRN

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3723 Collapse assay details

Data source: Panorama

Official Gene Symbol: Masp1
Peptide Sequence: SPEPISTQTHSVQILFR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 269
Peptide End: 285

CPTAC ID: non-CPTAC-3723
Peptide Molecular Mass: 1,939.0163
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y15 (2+)	12.3	3.5	2.5	12.5	4.6	4.5	17.5	5.8	5.1	15	15	15
y14 (2+)	4.9	2.1	1.5	9.4	3.2	4.1	10.6	3.8	4.4	15	15	15
b3 (1+)	9.4	2.7	1.3	10.8	4.5	3.8	14.3	5.2	4	15	15	15
y14 (3+)	10.3	1.8	2.3	16.6	2.3	3.8	19.5	2.9	4.4	15	15	15
y12 (2+)	12.4	3.6	1.4	16	3.9	3.5	20.2	5.3	3.8	15	15	15
sum	3	1.7	0.8	8.1	3	3.6	8.6	3.4	3.7	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-2682 Collapse assay details

Data source: Panorama

Official Gene Symbol: MASP1
Peptide Sequence: TGVITSPDFPNPYPK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 194
Peptide End: 208

CPTAC ID: non-CPTAC-2682
Peptide Molecular Mass: 1,631.8195
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers