STK33, CPTAC-2946 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
STK33	N/A

Sequence Length (AA)	Molecular Weight (Da)
514	57831

Protein Name
Serine/threonine-protein kinase 33

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MADSGLDKKS	TKCPDCSSAS	QKDVLCVCSS	KTRVPPVLVV	EMSQTSSIGS
60	70	80	90	100
AESLISLERK	KEKNINRDIT	SRKDLPSRTS	NVERKASQQQ	WGRGNFTEGK
110	120	130	140	150
VPHIRIENGA	AIEEIYTFGR	ILGKGSFGIV	IEATDKETET	KWAIKKVNKE
160	170	180	190	200
KAGSSAVKLL	EREVNILKSV	KHEHIIHLEQ	VFETPKKMYL	VMELCEDGEL
210	220	230	240	250
KEILDRKGHF	SENETRWIIQ	SLASAIAYLH	NNDIVHRDLK	LENIMVKSSL
260	270	280	290	300
IDDNNEINLN	IKVTDFGLAV	KKQSRSEAML	QATCGTPIYM	APEVISAHDY
310	320	330	340	350
SQQCDIWSIG	VVMYMLLRGE	PPFLASSEEK	LFELIRKGEL	HFENAVWNSI
360	370	380	390	400
SDCAKSVLKQ	LMKVDPAHRI	TAKELLDNQW	LTGNKLSSVR	PTNVLEMMKE
410	420	430	440	450
WKNNPESVEE	NTTEEKNKPS	TEEKLKSYQP	WGNVPDANYT	SDEEEEKQST
460	470	480	490	500
AYEKQFPATS	KDNFDMCSSS	FTSSKLLPAE	IKGEMEKTPV	TPSQGTATKY
510	514
PAKSGALSRT	KKKL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-2946 Collapse assay details

Data source: Panorama

Official Gene Symbol: STK33
Peptide Sequence: ELLDNQWLTGNK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 374
Peptide End: 385

CPTAC ID: non-CPTAC-2946
Peptide Molecular Mass: 1,429.7201
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	6.2	4.8	4.3	7.2	5.3	4.8	9.5	7.2	6.4	15	15	15
y4 (1+)	5.3	5.6	4.7	7.3	5.6	5	9	7.9	6.9	15	15	15
y5 (1+)	5.3	5.8	3.6	7.8	5.2	4.2	9.4	7.8	5.5	15	15	15
sum	5	5.2	4.2	6.9	5.3	4.5	8.5	7.4	6.2	15	15	15

STK33

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-2946 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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STK33

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-2946 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments