NUAK2, CPTAC-2947 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
NUAK2	OMPHK2, SNARK

Sequence Length (AA)	Molecular Weight (Da)
628	69612

Protein Name
NUAK family SNF1-like kinase 2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MESLVFARRS	GPTPSAAELA	RPLAEGLIKS	PKPLMKKQAV	KRHHHKHNLR
60	70	80	90	100
HRYEFLETLG	KGTYGKVKKA	RESSGRLVAI	KSIRKDKIKD	EQDLMHIRRE
110	120	130	140	150
IEIMSSLNHP	HIIAIHEVFE	NSSKIVIVME	YASRGDLYDY	ISERQQLSER
160	170	180	190	200
EARHFFRQIV	SAVHYCHQNR	VVHRDLKLEN	ILLDANGNIK	IADFGLSNLY
210	220	230	240	250
HQGKFLQTFC	GSPLYASPEI	VNGKPYTGPE	VDSWSLGVLL	YILVHGTMPF
260	270	280	290	300
DGHDHKILVK	QISNGAYREP	PKPSDACGLI	RWLLMVNPTR	RATLEDVASH
310	320	330	340	350
WWVNWGYATR	VGEQEAPHEG	GHPGSDSARA	SMADWLRRSS	RPLLENGAKV
360	370	380	390	400
CSFFKQHAPG	GGSTTPGLER	QHSLKKSRKE	NDMAQSLHSD	TADDTAHRPG
410	420	430	440	450
KSNLKLPKGI	LKKKVSASAE	GVQEDPPELS	PIPASPGQAA	PLLPKKGILK
460	470	480	490	500
KPRQRESGYY	SSPEPSESGE	LLDAGDVFVS	GDPKEQKPPQ	ASGLLLHRKG
510	520	530	540	550
ILKLNGKFSQ	TALELAAPTT	FGSLDELAPP	RPLARASRPS	GAVSEDSILS
560	570	580	590	600
SESFDQLDLP	ERLPEPPLRG	CVSVDNLTGL	EEPPSEGPGS	CLRRWRQDPL
610	620	628
GDSCFSLTDC	QEVTATYRQA	LRVCSKLT

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-2947 Collapse assay details

Data source: Panorama

Official Gene Symbol: NUAK2
Peptide Sequence: EQKPPQASGLLLHR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 485
Peptide End: 498

CPTAC ID: non-CPTAC-2947
Peptide Molecular Mass: 1,572.8736
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	7.4	7.2	5.6	9.8	6.8	6.9	12.3	9.9	8.9	15	15	15
y3 (1+)	2.9	5.8	4	9.8	5.4	6	10.2	7.9	7.2	15	15	15
y4 (1+)	3	5.5	4.9	11.2	6	5.1	11.6	8.1	7.1	15	15	15
sum	3.2	5.7	4.3	10.2	5.6	5.5	10.7	8	7	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for CPTAC-2844 Collapse assay details

Data source: Panorama

Official Gene Symbol: NUAK2
Peptide Sequence: IADFGLSNLYHQGK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 191
Peptide End: 204

CPTAC ID: CPTAC-2844
Peptide Molecular Mass: 1,561.7889
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend