ICK, CPTAC-2977 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
ICK	KIAA0936

Sequence Length (AA)	Molecular Weight (Da)
632	71427

Protein Name
Serine/threonine-protein kinase ICK

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MNRYTTIRQL	GDGTYGSVLL	GRSIESGELI	AIKKMKRKFY	SWEECMNLRE
60	70	80	90	100
VKSLKKLNHA	NVVKLKEVIR	ENDHLYFIFE	YMKENLYQLI	KERNKLFPES
110	120	130	140	150
AIRNIMYQIL	QGLAFIHKHG	FFHRDLKPEN	LLCMGPELVK	IADFGLAREI
160	170	180	190	200
RSKPPYTDYV	STRWYRAPEV	LLRSTNYSSP	IDVWAVGCIM	AEVYTLRPLF
210	220	230	240	250
PGASEIDTIF	KICQVLGTPK	KTDWPEGYQL	SSAMNFRWPQ	CVPNNLKTLI
260	270	280	290	300
PNASSEAVQL	LRDMLQWDPK	KRPTASQALR	YPYFQVGHPL	GSTTQNLQDS
310	320	330	340	350
EKPQKGILEK	AGPPPYIKPV	PPAQPPAKPH	TRISSRQHQA	SQPPLHLTYP
360	370	380	390	400
YKAEVSRTDH	PSHLQEDKPS	PLLFPSLHNK	HPQSKITAGL	EHKNGEIKPK
410	420	430	440	450
SRRRWGLISR	STKDSDDWAD	LDDLDFSPSL	SRIDLKNKKR	QSDDTLCRFE
460	470	480	490	500
SVLDLKPSEP	VGTGNSAPTQ	TSYQRRDTPT	LRSAAKQHYL	KHSRYLPGIS
510	520	530	540	550
IRNGILSNPG	KEFIPPNPWS	SSGLSGKSSG	TMSVISKVNS	VGSSSTSSSG
560	570	580	590	600
LTGNYVPSFL	KKEIGSAMQR	VHLAPIPDPS	PGYSSLKAMR	PHPGRPFFHT
610	620	630	632
QPRSTPGLIP	RPPAAQPVHG	RTDWASKYAS	RR

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-2977 Collapse assay details

Data source: Panorama

Official Gene Symbol: ICK
Peptide Sequence: QHQASQPPLHLTYPYK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 337
Peptide End: 352

CPTAC ID: non-CPTAC-2977
Peptide Molecular Mass: 1,906.9690
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b3 (1+)	18.1	6.6	4.8	23.7	7.7	6.2	29.8	10.1	7.8	15	15	15
y3 (1+)	16.6	6.2	4.9	25.2	8.3	6.5	30.2	10.4	8.1	15	15	15
y6 (1+)	47.6	8.3	5	66.6	8.3	5.8	81.9	11.7	7.7	15	15	15
sum	17.4	6	4.5	27.6	7.1	5.6	32.6	9.3	7.2	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-2976 Collapse assay details

Data source: Panorama

Official Gene Symbol: ICK
Peptide Sequence: QLGDGTYGSVLLGR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 9
Peptide End: 22

CPTAC ID: non-CPTAC-2976
Peptide Molecular Mass: 1,434.7467
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend