PHKG1, CPTAC-3008 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
PHKG1	PHKG

Sequence Length (AA)	Molecular Weight (Da)
387	45024

Protein Name
Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MTRDEALPDS	HSAQDFYENY	EPKEILGRGV	SSVVRRCIHK	PTSQEYAVKV
60	70	80	90	100
IDVTGGGSFS	PEEVRELREA	TLKEVDILRK	VSGHPNIIQL	KDTYETNTFF
110	120	130	140	150
FLVFDLMKRG	ELFDYLTEKV	TLSEKETRKI	MRALLEVICT	LHKLNIVHRD
160	170	180	190	200
LKPENILLDD	NMNIKLTDFG	FSCQLEPGER	LREVCGTPSY	LAPEIIECSM
210	220	230	240	250
NEDHPGYGKE	VDMWSTGVIM	YTLLAGSPPF	WHRKQMLMLR	MIMSGNYQFG
260	270	280	290	300
SPEWDDYSDT	VKDLVSRFLV	VQPQNRYTAE	EALAHPFFQQ	YLVEEVRHFS
310	320	330	340	350
PRGKFKVIAL	TVLASVRIYY	QYRRVKPVTR	EIVIRDPYAL	RPLRRLIDAY
360	370	380	387
AFRIYGHWVK	KGQQQNRAAL	FENTPKAVLL	SLAEEDY

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3008 Collapse assay details

Data source: Panorama

Official Gene Symbol: PHKG1
Peptide Sequence: AALFENTPK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 368
Peptide End: 376

CPTAC ID: non-CPTAC-3008
Peptide Molecular Mass: 989.5182
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	9.1	7.7	5.2	9.6	5.8	7	13.2	9.6	8.7	15	15	15
y7 (1+)	7.2	7.7	6.4	9.1	7	6.5	11.6	10.4	9.1	15	15	15
y6 (1+)	7.4	6.5	6.2	8.6	5.6	7.1	11.3	8.6	9.4	15	15	15
sum	7.7	6.9	5.7	8.8	5.7	6.8	11.7	8.9	8.9	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-3007 Collapse assay details

Data source: Panorama

Official Gene Symbol: PHKG1
Peptide Modified Sequence: LTDFGFSC[+57.0]QLEPGER
Modification Type: Carbamidomethyl (C)
Protein - Site of Modification: 174
Peptide - Site of Modification: 8
Peptide Start: 166
Peptide End: 180

CPTAC ID: non-CPTAC-3007
Peptide Molecular Mass: 1,754.7934
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend