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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Arid5a N/A
Sequence Length (AA) Molecular Weight (Da)
590 63898
Protein Name
AT-rich interactive domain-containing protein 5A
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAAPPAKGNT EQSEEGDLPQ LPVSPKPDDE QSRSQSPTQL QDSPEAGGEQ
60 70 80 90 100
EEEQAFLVSL YKFMKERHTP IERVPHLGFK QINLWKIYKA VEKLGAYELV
110 120 130 140 150
TGRRLWKNVY DELGGSPGST SAATCTRRHY ERLVLPYVRH LKGEDDKPLP
160 170 180 190 200
PTKPRKQYKM AKELRGDDGT TEKLKKAKDS EERRVEQTTP GKTKSDATGQ
210 220 230 240 250
TQLPCQGSSR DSTEQLGPVS GPSPPLTGAS SCPEAYKRLL SSFYCKGAHG
260 270 280 290 300
IMSPLAKKKL LAQVSKAEAL QCQEEGCRHG ARSPNKDIQD SPQNLRGPAE
310 320 330 340 350
NSEHQLTPRE GLQAPGGSTR MEAQVGPCPT APMFSGCFHA YPTEVLKPVS
360 370 380 390 400
QHPRDFFSGL KDRVLLGPPG KEEGPTTKES HLVWGGDANH PSAFHKGSTR
410 420 430 440 450
KRSFYPKPKA CWVSPMAKVP TERPGAPSPH PSSPGLGSKR GLEEEGFAHG
460 470 480 490 500
GKKLRAVSPF LKEVDSKETG GKPAAPGLAV SCLLGPTPGP TPPEAYRGTM
510 520 530 540 550
LRCPLNFTGS ADPLKGQASL PFSPLVIPAF PAHLLATTGS SPMAASLMHF
560 570 580 590
PPTPYDAVLR NRLGPASSAW HMPPVTTYAA PHFFHLNTKL

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3309 Collapse assay details

Data source: Panorama

Official Gene Symbol
Arid5a
Peptide Sequence
GPAENSEHQLTPR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
296
Peptide End
309
CPTAC ID
non-CPTAC-3309
Peptide Molecular Mass
1,434.6852
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (2+) 11.7 6.5 4.6 18.7 9.4 7.2 22.1 11.4 8.5 15 15 15
y5 (1+) 10.4 5.4 7.1 18.5 10.5 9.1 21.2 11.8 11.5 15 15 15
y12 (3+) 13.9 8 4.5 17.1 12 5.9 22 14.4 7.4 15 15 15
y10 (2+) 13.1 5 5.6 18.5 8.1 5.1 22.7 9.5 7.6 15 15 15
y11 (2+) 6.5 4.9 4.9 17.1 9.1 6 18.3 10.3 7.7 15 15 15
sum 5 2.8 2 14.2 8.8 3.4 15.1 9.2 3.9 15 15 15


Additional Resources and Comments