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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Col1a2

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Col1a2 Cola2
Sequence Length (AA) Molecular Weight (Da)
1372 129557
Protein Name
Collagen alpha-2(I) chain
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MLSFVDTRTL LLLAVTSCLA TCQYLQSGSV RKGPTGDRGP RGQRGPAGPR
60 70 80 90 100
GRDGVDGPMG PPGPPGSPGP PGSPAPPGLT GNFAAQYSDK GVSSGPGPMG
110 120 130 140 150
LMGPRGPPGA VGAPGPQGFQ GPAGEPGEPG QTGPAGPRGP AGSPGKAGED
160 170 180 190 200
GHPGKPGRPG ERGVVGPQGA RGFPGTPGLP GFKGVKGHSG MDGLKGQPGA
210 220 230 240 250
QGVKGEPGAP GENGTPGQAG ARGLPGERGR VGAPGPAGAR GSDGSVGPVG
260 270 280 290 300
PAGPIGSAGP PGFPGAPGPK GELGPVGNPG PAGPAGPRGE VGLPGLSGPV
310 320 330 340 350
GPPGNPGTNG LTGAKGATGL PGVAGAPGLP GPRGIPGPAG AAGATGARGL
360 370 380 390 400
VGEPGPAGSK GESGNKGEPG SVGAQGPPGP SGEEGKRGSP GEAGSAGPAG
410 420 430 440 450
PPGLRGSPGS RGLPGADGRA GVMGPPGNRG STGPAGIRGP NGDAGRPGEP
460 470 480 490 500
GLMGPRGLPG SPGNVGPSGK EGPVGLPGID GRPGPIGPAG PRGEAGNIGF
510 520 530 540 550
PGPKGPSGDP GKPGERGHPG LAGARGAPGP DGNNGAQGPP GPQGVQGGKG
560 570 580 590 600
EQGPAGPPGF QGLPGPSGTT GEVGKPGERG LPGEFGLPGP AGPRGERGTP
610 620 630 640 650
GESGAAGPSG PIGSRGPSGA PGPDGNKGEA GAVGAPGSAG ASGPGGLPGE
660 670 680 690 700
RGAAGIPGGK GEKGETGLRG DTGNTGRDGA RGIPGAVGAP GPAGASGDRG
710 720 730 740 750
EAGAAGPSGP AGPRGSPGER GEVGPAGPNG FAGPAGAAGQ PGAKGEKGTK
760 770 780 790 800
GPKGENGIVG PTGSVGAAGP SGPNGPPGPV GSRGDGGPPG MTGFPGAAGR
810 820 830 840 850
TGPPGPSGIA GPPGPPGAAG KEGIRGPRGD QGPVGRTGET GASGPPGFVG
860 870 880 890 900
EKGPSGEPGT AGAPGTAGPQ GLLGAPGILG LPGSRGERGL PGIAGALGEP
910 920 930 940 950
GPLGISGPPG ARGPPGAVGS PGVNGAPGEA GRDGNPGSDG PPGRDGQPGH
960 970 980 990 1000
KGERGYPGSI GPTGAAGAPG PHGSVGPAGK HGNRGEPGPA GSVGPVGAVG
1010 1020 1030 1040 1050
PRGPSGPQGI RGDKGEPGDK GHRGLPGLKG YSGLQGLPGL AGLHGDQGAP
1060 1070 1080 1090 1100
GPVGPAGPRG PAGPSGPVGK DGRSGQPGPV GPAGVRGSQG SQGPAGPPGP
1110 1120 1130 1140 1150
PGPPGPPGVS GGGYDFGFEG DFYRADQPRS QPSLRPKDYE VDATLKSLNN
1160 1170 1180 1190 1200
QIETLLTPEG SRKNPARTCR DLRLSHPEWN SDYYWIDPNQ GCTMDAIKVY
1210 1220 1230 1240 1250
CDFSTGETCI QAQPVNTPAK NSYSRAQANK HVWLGETING GSQFEYNVEG
1260 1270 1280 1290 1300
VSSKEMATQL AFMRLLANRA SQNITYHCKN SIAYLDEETG SLNKAVLLQG
1310 1320 1330 1340 1350
SNDVELVAEG NSRFTYSVLV DGCSKKTNEW GKTIIEYKTN KPSRLPFLDI
1360 1370 1372
APLDIGGADQ EFRVEVGPVC FK

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3310 Collapse assay details

Data source: Panorama

Official Gene Symbol
Col1a2
Peptide Sequence
GVVGPQGAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
162
Peptide End
171
CPTAC ID
non-CPTAC-3310
Peptide Molecular Mass
839.4614
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (2+) 6.7 3.5 2.8 17.5 10.8 5 18.7 11.4 5.7 15 15 15
y5 (1+) 6.9 4.6 3.4 17.7 9.3 5.7 19 10.4 6.6 15 15 15
y5 (2+) 9.7 4.2 3.9 13.2 10.6 5.5 16.4 11.4 6.7 15 15 15
y6 (1+) 4.4 5.5 3.1 16.1 10 5.1 16.7 11.4 6 15 15 15
y7 (1+) 7 5 5.4 19.2 9.6 6.4 20.4 10.8 8.4 15 15 15
sum 3.2 1.6 1.1 16 9.7 4.4 16.3 9.8 4.5 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments