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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Icam2 Icam-2
Sequence Length (AA) Molecular Weight (Da)
277 31390
Protein Name
Intercellular adhesion molecule 2
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSSFACWSLS LLILFYSPGS GEKAFEVYIW SEKQIVEATE SWKINCSTNC
60 70 80 90 100
AAPDMGGLET PTNKIMLEEH PQGKWKQFLV SNVSKDTVFF CHFTCSGKQH
110 120 130 140 150
SESLNIRVYQ PPAQVTLKLQ PPRVFVGEDF TIECTVSPVQ PLERLTLSLL
160 170 180 190 200
RGRETLKNQT FGGAETVPQE ATATFNSTAL KKDGLNFSCQ AELDLRPHGG
210 220 230 240 250
YIIRSISEYQ ILEVYEPMQD NQMVIIIVVV SILLFLFVTS VLLCFIFGQH
260 270 277
WHRRRTGTYG VLAAWRRLPR AFRARPV

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-677 Collapse assay details

Data source: Panorama

Official Gene Symbol
ICAM2
Peptide Modified Sequence
AAPAPQEATATFN[+1.0]STADR
Modification Type
Deamidated (NQ)
Protein - Site of Modification
176
Peptide - Site of Modification
13
Peptide Start
164
Peptide End
181
CPTAC ID
CPTAC-677
Peptide Molecular Mass
1,818.8384
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
serum
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive (ThermoFisher)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Dionex UltiMate 3000 RSLCnano LC (ThermoFisher)
Column Packing
Acclaim PepMap100 C18, 5um (Trap); Acclaim PepMap RSLC C18, 2um (Analytical)
Column Dimensions
300um x 5mm (Trap); 75um x 25cm (Analytical)
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y12 (1+) 15.4 18.7 19.4 15 17.7 20.8 21.5 25.7 28.4 15 15 15
y10 (1+) 14.1 15.6 17.9 14.9 14.4 15.7 20.5 21.2 23.8 15 15 15
y8 (1+) 23.3 20.5 20.9 32.5 34.7 26.6 40 40.3 33.8 15 15 15
sum 6.7 12.2 12.3 7.6 16.3 12.8 10.1 20.4 17.8 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3315 Collapse assay details

Data source: Panorama

Official Gene Symbol
Icam2
Peptide Sequence
LTLSLLR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
144
Peptide End
151
CPTAC ID
non-CPTAC-3315
Peptide Molecular Mass
814.5276
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 5.7 4.8 4.4 12.8 9 4.8 14 10.2 6.5 15 15 15
y4 (1+) 6.4 4.7 3.7 12.3 7.5 5.5 13.9 8.9 6.6 15 15 15
y3 (2+) 114.4 86.4 64.2 132.1 123.8 57.4 174.8 151 86.1 15 15 15
y5 (1+) 5.3 2.4 3.3 13.4 7.8 4.2 14.4 8.2 5.3 15 15 15
y6 (1+) 8.1 7.2 5.3 13.3 9.9 9.3 15.6 12.2 10.7 15 15 15
sum 1.8 1.6 1.6 11.9 7.4 3.9 12 7.6 4.2 15 15 15

Additional Resources and Comments