Icam2, CPTAC-3315 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Icam2	Icam-2

Sequence Length (AA)	Molecular Weight (Da)
277	31390

Protein Name
Intercellular adhesion molecule 2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSSFACWSLS	LLILFYSPGS	GEKAFEVYIW	SEKQIVEATE	SWKINCSTNC
60	70	80	90	100
AAPDMGGLET	PTNKIMLEEH	PQGKWKQFLV	SNVSKDTVFF	CHFTCSGKQH
110	120	130	140	150
SESLNIRVYQ	PPAQVTLKLQ	PPRVFVGEDF	TIECTVSPVQ	PLERLTLSLL
160	170	180	190	200
RGRETLKNQT	FGGAETVPQE	ATATFNSTAL	KKDGLNFSCQ	AELDLRPHGG
210	220	230	240	250
YIIRSISEYQ	ILEVYEPMQD	NQMVIIIVVV	SILLFLFVTS	VLLCFIFGQH
260	270	277
WHRRRTGTYG	VLAAWRRLPR	AFRARPV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-677 Collapse assay details

Data source: Panorama

Official Gene Symbol: ICAM2
Peptide Modified Sequence: AAPAPQEATATFN[+1.0]STADR
Modification Type: Deamidated (NQ)
Protein - Site of Modification: 176
Peptide - Site of Modification: 13
Peptide Start: 164
Peptide End: 181

CPTAC ID: CPTAC-677
Peptide Molecular Mass: 1,818.8384
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: N/A
Matrix: serum
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Q-Exactive (ThermoFisher)
Internal Standard: synthetic peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Dionex UltiMate 3000 RSLCnano LC (ThermoFisher)
Column Packing: Acclaim PepMap100 C18, 5um (Trap); Acclaim PepMap RSLC C18, 2um (Analytical)
Column Dimensions: 300um x 5mm (Trap); 75um x 25cm (Analytical)
Flow Rate: 0.5 uL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y12 (1+)	15.4	18.7	19.4	15	17.7	20.8	21.5	25.7	28.4	15	15	15
y10 (1+)	14.1	15.6	17.9	14.9	14.4	15.7	20.5	21.2	23.8	15	15	15
y8 (1+)	23.3	20.5	20.9	32.5	34.7	26.6	40	40.3	33.8	15	15	15
sum	6.7	12.2	12.3	7.6	16.3	12.8	10.1	20.4	17.8	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-3315 Collapse assay details

Data source: Panorama

Official Gene Symbol: Icam2
Peptide Sequence: LTLSLLR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 144
Peptide End: 151

CPTAC ID: non-CPTAC-3315
Peptide Molecular Mass: 814.5276
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

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Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.