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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Cdh5 N/A
Sequence Length (AA) Molecular Weight (Da)
784 87903
Protein Name
Cadherin-5
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MQRLTELATA LGAFLGLLAV AAMAGPNFPQ IDTPNMLPAH HRQKRDWIWN
60 70 80 90 100
QMHIDEEKNE SLPHYVGKIK SNVNRQNAKY VLQGEFAGKI FGVDANTGNV
110 120 130 140 150
LAYERLDREK VSEYFLTALI VDKNTNKNLE QPSSFTVKVH DINDNWPVFS
160 170 180 190 200
HQVFNASVPE MSAIGTSVIR VTAVDADDPT VAGHATVLYQ IVKGNEYFSI
210 220 230 240 250
DNSGLIFTKI KNLDREKQAE YKIVVETQDA LGLRGESGTA TVMIRLEDIN
260 270 280 290 300
DNFPVFTQST YTFSVPEDIR VGKPLGFLTV VDPDEPQNRM TKYSIMQGEY
310 320 330 340 350
RDTFTIETDP KRNEGIIKPT KSLDYEVIQQ YTFYIEATDP TIRYEYLSST
360 370 380 390 400
SGKNKAMVTI NVLDVDEPPV FQRHFYHFKL PENQKKPLIG TVVAKDPDKA
410 420 430 440 450
QRSIGYSIRK TSDRGQFFRI TKQGNIYNEK ELDRETYAWY NLTVEANELD
460 470 480 490 500
SRGNPVGKES IVQVYIEVLD ENDNPPEFAQ PYEPKVCENA AQGKLVVQIS
510 520 530 540 550
ATDKDVVPVN PKFKFALKNE DSNFTLINNH DNTANITVKY GQFNREHAKF
560 570 580 590 600
HYLPVLISDN GVPSLTGTST LTVGVCKCNE QGEFTFCEEM AAQAGVSIQA
610 620 630 640 650
LVAIFLCILT ITVITLLIIL RRRIRKQAHA HSKSALEIHE QLVTYDEEGG
660 670 680 690 700
GEMDTTSYDV SVLNSVRGGS TKPLRSTMDA RPAVYTQVQK PPRLAPGLHG
710 720 730 740 750
GPREMATMID VKKEEADNDG GGPPYDTLHI YGYEGAESIA ESLSSLSTNS
760 770 780 784
SDSDIDYDFL NDWGPRFKML AELYGSDPQE ELII

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3316 Collapse assay details

Data source: Panorama

Official Gene Symbol
Cdh5
Peptide Sequence
SIGYSIR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
402
Peptide End
409
CPTAC ID
non-CPTAC-3316
Peptide Molecular Mass
794.4287
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 18.7 5.7 6 18 7.6 6.8 26 9.5 9.1 15 15 15
y4 (1+) 8.3 5.2 5.2 13.9 8.7 5.6 16.2 10.1 7.6 15 15 15
y5 (1+) 3.9 4.6 4 13.4 9.3 5.2 14 10.4 6.6 15 15 15
b2 (1+) 6.3 6.9 7.9 15.2 8.6 7.4 16.5 11 10.8 15 15 15
y3 (1+) 27.4 6.8 6.2 45.8 11.5 8.2 53.4 13.4 10.3 15 15 15
sum 4 4.1 3.7 11.6 8.4 4.7 12.3 9.3 6 15 15 15

Additional Resources and Comments