C4bpa, CPTAC-3318 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
C4bpa	C4bp

Sequence Length (AA)	Molecular Weight (Da)
469	51524

Protein Name
C4b-binding protein

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MCAKQQQTLL	PTRAAHGRLH	RNRDAVAWPF	STLCRVSGPT	LFQMTFTAAL
60	70	80	90	100
WVAVFGKCGP	PPAIPNALPA	SDVNRTDFES	HTTLKYECLP	GYGRGISRMM
110	120	130	140	150
VYCKPSGEWE	ISVSCAKKHC	RNPGYLDNGY	VNGETITFGS	QIEFSCQEGF
160	170	180	190	200
ILVGSSTSSC	EVRGKGVAWS	NPFPECVIVK	CGPPPDISNG	KHSGTEDFYP
210	220	230	240	250
YNHGISYTCD	PGFRLVGSPF	IGCTVVNKTV	PVWSSSPPTC	EKIICSQPNI
260	270	280	290	300
LHGVIVSGYK	ATYTHRDSVR	LACLNGTVLR	GRHVIECQGN	GNWSSLPTCE
310	320	330	340	350
FDCDLPPAIV	NGYYTSMVYS	KITLVTYECD	KGYRLVGKAI	ISCSFSKWKG
360	370	380	390	400
TAPQCKALCQ	KPEVGNGTLS	DEKDQYVESE	NVTIQCDSGF	AMLGSQSISC
410	420	430	440	450
SESGTWYPEV	PRCEQEASED	LKPALTGNKT	MQYVPNSHDV	KMALEIYKLT
460	469
LEVELLQLQI	QKEKHTEAH

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for non-CPTAC-3318 Collapse assay details

Data source: Panorama

Official Gene Symbol: C4bpa
Peptide Sequence: TDFESHTTLK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 75
Peptide End: 85

CPTAC ID: non-CPTAC-3318
Peptide Molecular Mass: 1,177.5615
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	12.2	8.2	15.2	17.4	12.6	17.8	21.3	15	23.4	15	15	15
y6 (1+)	15.8	6.8	8.4	21	11.4	10.9	26.3	13.3	13.8	15	15	15
y9 (2+)	12.5	7.3	9.3	16.7	8.6	9.8	20.9	11.3	13.5	15	15	15
y8 (2+)	7	6.7	9.6	13.5	8.6	9	15.2	10.9	13.2	15	15	15
y6 (2+)	26	6.8	8.1	37.9	15.1	9.3	46	16.6	12.3	15	15	15
sum	4.8	3.9	4.8	12.9	6.9	5.1	13.8	7.9	7	15	15	15

C4bpa

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3318 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Search form

C4bpa

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3318 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments