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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Cfi If
Sequence Length (AA) Molecular Weight (Da)
603 67261
Protein Name
Complement factor I
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MKLAHLSLFL LALHLSSSRS PSASDLPQEE LVDQKCLLQK YTHRSCNKVF
60 70 80 90 100
CQPWQRCIEG TCICKLPYQC PRAGTPVCAM NGRSYPTYCH QKSFECLHPE
110 120 130 140 150
IKFSHNGTCA AEGKFNVSLI YGRTKTEGLV QVKLVDQDER MFICKNSWSM
160 170 180 190 200
AEANVACVDL GFPLGVRDIQ GSFNISGNLH INDTECLHVH CRGVETSLAE
210 220 230 240 250
CAFTKRRTEL SNGLAGVVCY KQDADFPTSL SFQCVNGKHI PQEKACNGVN
260 270 280 290 300
DCGDQSDELC CKGCRGNASL CKSGVCIPDQ YKCNGEVDCI TGEDESRCEE
310 320 330 340 350
DRQQNIPKGL ARSAQGEAEI ETEETEMLTP GMDNERKRIK SLLPKLSCGV
360 370 380 390 400
KRNTHTRRKR VIGGKPANVG DYPWQVAIKD GQRITCGGIY IGGCWILTAA
410 420 430 440 450
HCVRPSRAHS YQVWTALLDW LKPNSQLGIQ TVKRVIVHEK YNGATFQNDI
460 470 480 490 500
ALIEMKMHTG KKECELPNSV PACVPWSPYL FQPNDRCIIS GWGRGKDNQK
510 520 530 540 550
VYSLRWGEVD LIGNCSQFYP DRYYEKEMQC AGTRDGSIDA CKGDSGGPLV
560 570 580 590 600
CEDINNVTYV WGIVSWGENC GKPEFPGVYT RVANYFDWIS YHVGRSLVSQ
603
HNV

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-1113 Collapse assay details

Data source: Panorama

Official Gene Symbol
CFI
Peptide Sequence
HGNTDSEGIVEVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
118
Peptide End
130
CPTAC ID
non-CPTAC-1113
Peptide Molecular Mass
1,383.6630
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b5 (1+) 19.4 10.4 9.5 47.1 10.5 11.7 50.9 14.8 15.1 15 15 15
y10 (3+) 26.8 36 9.1 79.3 70 22.2 83.7 78.7 24 7 8 8
y2 (1+) 25.1 10.2 10.4 60 24.4 11.7 65 26.4 15.7 13 15 15
y4 (1+) 13.5 5.7 6.3 46.9 9.1 7.5 48.8 10.7 9.8 15 15 15
y6 (1+) 10.4 6.4 6.5 20.7 7.7 6.2 23.2 10 9 15 15 15
sum 13.3 10.5 8.7 37.3 14.1 8.3 39.6 17.6 12 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-2659 Collapse assay details

Data source: Panorama

Official Gene Symbol
CFI
Peptide Sequence
IVIEYVDR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
407
Peptide End
414
CPTAC ID
non-CPTAC-2659
Peptide Molecular Mass
1,005.5495
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (2+) 12.7 6.2 6.3 15.6 11.6 10.1 20.1 13.2 11.9 15 15 15
y5 (1+) 12.9 6.8 8.7 18.1 10.1 10.4 22.2 12.2 13.6 15 15 15
y6 (1+) 7.6 7.7 6.9 13.1 9.5 9.5 15.1 12.2 11.7 15 15 15
b3 (1+) 32.4 6.2 6.7 35.4 7.9 9.7 48 10 11.8 15 15 15
y4 (1+) 13.6 6.3 5.9 11.9 7.3 6.1 18.1 9.6 8.5 15 15 15
sum 5.6 4.8 5.7 12.3 7.9 8.2 13.5 9.2 10 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3319 Collapse assay details

Data source: Panorama

Official Gene Symbol
Cfi
Peptide Sequence
TEGLVQVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
125
Peptide End
133
CPTAC ID
non-CPTAC-3319
Peptide Molecular Mass
872.4967
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 6.3 4.6 4.8 18 7.2 6.8 19.1 8.5 8.3 15 15 15
y4 (1+) 10.9 4.2 5.9 19.3 9.9 12.8 22.2 10.8 14.1 15 15 15
y2 (1+) 29.3 16.8 5.6 137.7 34 9.1 140.8 37.9 10.7 15 15 15
b4 (2+) 79 67 82.4 92.9 70 81.2 121.9 96.9 115.7 15 15 15
b2 (1+) 4.9 5.5 3.7 15.3 10.1 8.9 16.1 11.5 9.6 15 15 15
sum 16.5 2.9 1.9 56.6 6.9 6.6 59 7.5 6.9 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-2660 Collapse assay details

Data source: Panorama

Official Gene Symbol
CFI
Peptide Sequence
VFSLQWGEVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
481
Peptide End
490
CPTAC ID
non-CPTAC-2660
Peptide Molecular Mass
1,191.6288
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 6.8 6.2 6.9 12.3 6.9 9.2 14.1 9.3 11.5 15 15 15
y2 (1+) 37.1 14.1 7.1 48.6 19 9.5 61.1 23.7 11.9 15 15 15
y6 (1+) 15.6 6.2 8.3 17.9 7.6 9.3 23.7 9.8 12.5 15 15 15
y9 (2+) 21.6 13.7 5.4 30.8 13.8 5.8 37.6 19.4 7.9 15 15 15
y8 (1+) 10.9 7.5 8.6 16.3 7 9.8 19.6 10.3 13 15 15 15
sum 9.4 3.5 5.9 12.1 5.2 7.9 15.3 6.3 9.9 15 15 15

Additional Resources and Comments