Overview Data source: UniProt

Official Gene Symbol Other Aliases
CFI IF
Sequence Length (AA) Molecular Weight (Da)
583 65750
Protein Name
Complement factor I
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MKLLHVFLLF LCFHLRFCKV TYTSQEDLVE KKCLAKKYTH LSCDKVFCQP
60 70 80 90 100
WQRCIEGTCV CKLPYQCPKN GTAVCATNRR SFPTYCQQKS LECLHPGTKF
110 120 130 140 150
LNNGTCTAEG KFSVSLKHGN TDSEGIVEVK LVDQDKTMFI CKSSWSMREA
160 170 180 190 200
NVACLDLGFQ QGADTQRRFK LSDLSINSTE CLHVHCRGLE TSLAECTFTK
210 220 230 240 250
RRTMGYQDFA DVVCYTQKAD SPMDDFFQCV NGKYISQMKA CDGINDCGDQ
260 270 280 290 300
SDELCCKACQ GKGFHCKSGV CIPSQYQCNG EVDCITGEDE VGCAGFASVT
310 320 330 340 350
QEETEILTAD MDAERRRIKS LLPKLSCGVK NRMHIRRKRI VGGKRAQLGD
360 370 380 390 400
LPWQVAIKDA SGITCGGIYI GGCWILTAAH CLRASKTHRY QIWTTVVDWI
410 420 430 440 450
HPDLKRIVIE YVDRIIFHEN YNAGTYQNDI ALIEMKKDGN KKDCELPRSI
460 470 480 490 500
PACVPWSPYL FQPNDTCIVS GWGREKDNER VFSLQWGEVK LISNCSKFYG
510 520 530 540 550
NRFYEKEMEC AGTYDGSIDA CKGDSGGPLV CMDANNVTYV WGVVSWGENC
560 570 580 583
GKPEFPGVYT KVANYFDWIS YHVGRPFISQ YNV

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-1113 Collapse assay details

Data source: Panorama

Official Gene Symbol
CFI
Peptide Sequence
HGNTDSEGIVEVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
118
Peptide End
130
CPTAC ID
non-CPTAC-1113
Peptide Molecular Mass
1,383.6630
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b5 (1+) 19.4 10.4 9.5 47.1 10.5 11.7 50.9 14.8 15.1 15 15 15
y10 (3+) 26.8 36 9.1 79.3 70 22.2 83.7 78.7 24 7 8 8
y2 (1+) 25.1 10.2 10.4 60 24.4 11.7 65 26.4 15.7 13 15 15
y4 (1+) 13.5 5.7 6.3 46.9 9.1 7.5 48.8 10.7 9.8 15 15 15
y6 (1+) 10.4 6.4 6.5 20.7 7.7 6.2 23.2 10 9 15 15 15
sum 13.3 10.5 8.7 37.3 14.1 8.3 39.6 17.6 12 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-2659 Collapse assay details

Data source: Panorama

Official Gene Symbol
CFI
Peptide Sequence
IVIEYVDR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
407
Peptide End
414
CPTAC ID
non-CPTAC-2659
Peptide Molecular Mass
1,005.5495
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (2+) 12.7 6.2 6.3 15.6 11.6 10.1 20.1 13.2 11.9 15 15 15
y5 (1+) 12.9 6.8 8.7 18.1 10.1 10.4 22.2 12.2 13.6 15 15 15
y6 (1+) 7.6 7.7 6.9 13.1 9.5 9.5 15.1 12.2 11.7 15 15 15
b3 (1+) 32.4 6.2 6.7 35.4 7.9 9.7 48 10 11.8 15 15 15
y4 (1+) 13.6 6.3 5.9 11.9 7.3 6.1 18.1 9.6 8.5 15 15 15
sum 5.6 4.8 5.7 12.3 7.9 8.2 13.5 9.2 10 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-2660 Collapse assay details

Data source: Panorama

Official Gene Symbol
CFI
Peptide Sequence
VFSLQWGEVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
481
Peptide End
490
CPTAC ID
non-CPTAC-2660
Peptide Molecular Mass
1,191.6288
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 6.8 6.2 6.9 12.3 6.9 9.2 14.1 9.3 11.5 15 15 15
y2 (1+) 37.1 14.1 7.1 48.6 19 9.5 61.1 23.7 11.9 15 15 15
y6 (1+) 15.6 6.2 8.3 17.9 7.6 9.3 23.7 9.8 12.5 15 15 15
y9 (2+) 21.6 13.7 5.4 30.8 13.8 5.8 37.6 19.4 7.9 15 15 15
y8 (1+) 10.9 7.5 8.6 16.3 7 9.8 19.6 10.3 13 15 15 15
sum 9.4 3.5 5.9 12.1 5.2 7.9 15.3 6.3 9.9 15 15 15

Additional Resources and Comments