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Overview Data source: UniProt

Official Gene Symbol Other Aliases
CFB BF,BFD
Sequence Length (AA) Molecular Weight (Da)
764 85533
Protein Name
Complement factor B
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGSNLSPQLC LMPFILGLLS GGVTTTPWSL ARPQGSCSLE GVEIKGGSFR
60 70 80 90 100
LLQEGQALEY VCPSGFYPYP VQTRTCRSTG SWSTLKTQDQ KTVRKAECRA
110 120 130 140 150
IHCPRPHDFE NGEYWPRSPY YNVSDEISFH CYDGYTLRGS ANRTCQVNGR
160 170 180 190 200
WSGQTAICDN GAGYCSNPGI PIGTRKVGSQ YRLEDSVTYH CSRGLTLRGS
210 220 230 240 250
QRRTCQEGGS WSGTEPSCQD SFMYDTPQEV AEAFLSSLTE TIEGVDAEDG
260 270 280 290 300
HGPGEQQKRK IVLDPSGSMN IYLVLDGSDS IGASNFTGAK KCLVNLIEKV
310 320 330 340 350
ASYGVKPRYG LVTYATYPKI WVKVSEADSS NADWVTKQLN EINYEDHKLK
360 370 380 390 400
SGTNTKKALQ AVYSMMSWPD DVPPEGWNRT RHVIILMTDG LHNMGGDPIT
410 420 430 440 450
VIDEIRDLLY IGKDRKNPRE DYLDVYVFGV GPLVNQVNIN ALASKKDNEQ
460 470 480 490 500
HVFKVKDMEN LEDVFYQMID ESQSLSLCGM VWEHRKGTDY HKQPWQAKIS
510 520 530 540 550
VIRPSKGHES CMGAVVSEYF VLTAAHCFTV DDKEHSIKVS VGGEKRDLEI
560 570 580 590 600
EVVLFHPNYN INGKKEAGIP EFYDYDVALI KLKNKLKYGQ TIRPICLPCT
610 620 630 640 650
EGTTRALRLP PTTTCQQQKE ELLPAQDIKA LFVSEEEKKL TRKEVYIKNG
660 670 680 690 700
DKKGSCERDA QYAPGYDKVK DISEVVTPRF LCTGGVSPYA DPNTCRGDSG
710 720 730 740 750
GPLIVHKRSR FIQVGVISWG VVDVCKNQKR QKQVPAHARD FHINLFQVLP
760 764
WLKEKLQDED LGFL

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-1112 Collapse assay details

Data source: Panorama

Official Gene Symbol
CFB
Peptide Sequence
EELLPAQDIK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
620
Peptide End
629
CPTAC ID
non-CPTAC-1112
Peptide Molecular Mass
1,154.6183
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b4 (1+) 2.8 8.4 5 6.6 9.6 6.1 7.2 12.8 7.9 6 15 15
b3 (1+) 20.3 9.5 5 21.2 11.1 7.2 29.4 14.6 8.8 15 15 15
b2 (1+) 15.4 7.6 5.8 17.3 7.5 6.5 23.2 10.7 8.7 15 15 15
y6 (1+) 10.8 6 5.3 13.4 9.2 7.7 17.2 11 9.3 15 15 15
y7 (1+) 17.1 9.1 7.9 17.1 8.2 6.1 24.2 12.2 10 15 15 15
sum 8.7 4.5 2.4 11.1 6.3 4.2 14.1 7.7 4.8 15 15 15

Additional Resources and Comments