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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Cfb

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Cfb Bf, H2-Bf
Sequence Length (AA) Molecular Weight (Da)
761 85004
Protein Name
Complement factor B
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MESPQLCLVL LVLGFSSGGV SATPVLEARP QVSCSLEGVE IKGGSFQLLQ
60 70 80 90 100
GGQALEYLCP SGFYPYPVQT RTCRSTGSWS DLQTRDQKIV QKAECRAIRC
110 120 130 140 150
PRPQDFENGE FWPRSPFYNL SDQISFQCYD GYVLRGSANR TCQENGRWDG
160 170 180 190 200
QTAICDDGAG YCPNPGIPIG TRKVGSQYRL EDIVTYHCSR GLVLRGSQKR
210 220 230 240 250
KCQEGGSWSG TEPSCQDSFM YDSPQEVAEA FLSSLTETIE GADAEDGHSP
260 270 280 290 300
GEQQKRKIVL DPSGSMNIYL VLDGSDSIGS SNFTGAKRCL TNLIEKVASY
310 320 330 340 350
GVRPRYGLLT YATVPKVLVR VSDERSSDAD WVTEKLNQIS YEDHKLKSGT
360 370 380 390 400
NTKRALQAVY SMMSWAGDAP PEGWNRTRHV IIIMTDGLHN MGGNPVTVIQ
410 420 430 440 450
DIRALLDIGR DPKNPREDYL DVYVFGVGPL VDSVNINALA SKKDNEHHVF
460 470 480 490 500
KVKDMEDLEN VFYQMIDETK SLSLCGMVWE HKKGNDYHKQ PWQAKISVTR
510 520 530 540 550
PLKGHETCMG AVVSEYFVLT AAHCFMVDDQ KHSIKVSVGG QRRDLEIEEV
560 570 580 590 600
LFHPKYNING KKAEGIPEFY DYDVALVKLK NKLKYGQTLR PICLPCTEGT
610 620 630 640 650
TRALRLPQTA TCKQHKEQLL PVKDVKALFV SEQGKSLTRK EVYIKNGDKK
660 670 680 690 700
ASCERDATKA QGYEKVKDAS EVVTPRFLCT GGVDPYADPN TCKGDSGGPL
710 720 730 740 750
IVHKRSRFIQ VGVISWGVVD VCRDQRRQQL VPSYARDFHI NLFQVLPWLK
760 761
DKLKDEDLGF L

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3901 Collapse assay details

Data source: Panorama

Official Gene Symbol
Cfb
Peptide Sequence
DLEIEEVLFHPK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
544
Peptide End
555
CPTAC ID
non-CPTAC-3901
Peptide Molecular Mass
1,467.7609
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 6.4 4.5 2 7.9 11 12.4 10.2 11.9 12.6 15 15 15
y2 (1+) 6.3 3 1.9 7.6 13 13.7 9.9 13.3 13.8 15 15 15
y10 (2+) 3.6 1.4 1.6 8.2 12.2 14.4 9 12.3 14.5 15 15 15
y9 (2+) 6 2.9 2.5 10.2 12.2 14.5 11.8 12.5 14.7 15 15 15
b2 (1+) 5.9 2.6 1.7 9.6 14.2 14.6 11.3 14.4 14.7 15 15 15
sum 3 1.2 0.8 7 12.3 14.1 7.6 12.4 14.1 15 15 15

Additional Resources and Comments

Resources
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View Peptide in Panorama
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Comments

Assay Details for non-CPTAC-1112 Collapse assay details

Data source: Panorama

Official Gene Symbol
CFB
Peptide Sequence
EELLPAQDIK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
620
Peptide End
629
CPTAC ID
non-CPTAC-1112
Peptide Molecular Mass
1,154.6183
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b4 (1+) 2.8 8.4 5 6.6 9.6 6.1 7.2 12.8 7.9 6 15 15
b3 (1+) 20.3 9.5 5 21.2 11.1 7.2 29.4 14.6 8.8 15 15 15
b2 (1+) 15.4 7.6 5.8 17.3 7.5 6.5 23.2 10.7 8.7 15 15 15
y6 (1+) 10.8 6 5.3 13.4 9.2 7.7 17.2 11 9.3 15 15 15
y7 (1+) 17.1 9.1 7.9 17.1 8.2 6.1 24.2 12.2 10 15 15 15
sum 8.7 4.5 2.4 11.1 6.3 4.2 14.1 7.7 4.8 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments