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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Bche

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Bche N/A
Sequence Length (AA) Molecular Weight (Da)
603 68462
Protein Name
Cholinesterase
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MQTQHTKVTQ THFLLWILLL CMPFGKSHTE EDFIITTKTG RVRGLSMPVL
60 70 80 90 100
GGTVTAFLGI PYAQPPLGSL RFKKPQPLNK WPDIHNATQY ANSCYQNIDQ
110 120 130 140 150
AFPGFQGSEM WNPNTNLSED CLYLNVWIPV PKPKNATVMV WIYGGGFQTG
160 170 180 190 200
TSSLPVYDGK FLARVERVIV VSMNYRVGAL GFLAFPGNPD APGNMGLFDQ
210 220 230 240 250
QLALQWVQRN IAAFGGNPKS ITIFGESAGA ASVSLHLLCP QSYPLFTRAI
260 270 280 290 300
LESGSSNAPW AVKHPEEARN RTLTLAKFTG CSKENEMEMI KCLRSKDPQE
310 320 330 340 350
ILRNERFVLP SDSILSINFG PTVDGDFLTD MPHTLLQLGK VKKAQILVGV
360 370 380 390 400
NKDEGTAFLV YGAPGFSKDN DSLITRKEFQ EGLNMYFPGV SRLGKEAVLF
410 420 430 440 450
YYVDWLGEQS PEVYRDALDD VIGDYNIICP ALEFTKKFAE LENNAFFYFF
460 470 480 490 500
EHRSSKLPWP EWMGVMHGYE IEFVFGLPLG RRVNYTRAEE IFSRSIMKTW
510 520 530 540 550
ANFAKYGHPN GTQGNSTMWP VFTSTEQKYL TLNTEKSKIY SKLRAPQCQF
560 570 580 590 600
WRLFFPKVLE MTGDIDETEQ EWKAGFHRWS NYMMDWQNQF NDYTSKKESC
603
TAL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3902 Collapse assay details

Data source: Panorama

Official Gene Symbol
Bche
Peptide Sequence
YLTLNTEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
529
Peptide End
536
CPTAC ID
non-CPTAC-3902
Peptide Molecular Mass
980.5179
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b4 (2+) 128.1 126.1 116.3 166.7 121.8 121.2 210.2 175.3 168 15 15 15
b2 (1+) 8.4 4.4 5.4 11.1 5 7.5 13.9 6.7 9.2 15 15 15
y3 (1+) 39.7 11.2 6.1 56.7 12.3 7.9 69.2 16.6 10 15 15 15
y6 (1+) 16 3 2.4 13.3 8.1 5.7 20.8 8.6 6.2 15 15 15
y7 (1+) 24.5 11.3 8.4 23.8 11.2 9 34.2 15.9 12.3 15 15 15
sum 9.5 2.5 1.9 13 6.3 5.1 16.1 6.8 5.4 15 15 15

Additional Resources and Comments

Resources
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View Peptide in Panorama
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Comments

Assay Details for non-CPTAC-2645 Collapse assay details

Data source: Panorama

Official Gene Symbol
BCHE
Peptide Sequence
YLTLNTESTR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
528
Peptide End
537
CPTAC ID
non-CPTAC-2645
Peptide Molecular Mass
1,196.6037
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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view larger image
view larger image

Response Curves

Data source: Panorama

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view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 10.8 6.9 4.8 13.8 11.1 7.9 17.5 13.1 9.2 15 15 15
b2 (1+) 12.1 6.2 4.9 17.1 7.7 9.4 20.9 9.9 10.6 15 15 15
y8 (1+) 14.1 6.4 5.3 13.9 8.2 7 19.8 10.4 8.8 15 15 15
y7 (1+) 10.5 5.7 8.1 16.8 9.7 8 19.8 11.3 11.4 15 15 15
b3 (1+) 9.3 7.6 8.6 11.9 12.9 9.2 15.1 15 12.6 15 15 15
sum 5.3 4.3 4.6 9.4 8.1 7.3 10.8 9.2 8.6 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments