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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

BCHE

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
BCHE CHE1
Sequence Length (AA) Molecular Weight (Da)
602 68418
Protein Name
Cholinesterase
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MHSKVTIICI RFLFWFLLLC MLIGKSHTED DIIIATKNGK VRGMNLTVFG
60 70 80 90 100
GTVTAFLGIP YAQPPLGRLR FKKPQSLTKW SDIWNATKYA NSCCQNIDQS
110 120 130 140 150
FPGFHGSEMW NPNTDLSEDC LYLNVWIPAP KPKNATVLIW IYGGGFQTGT
160 170 180 190 200
SSLHVYDGKF LARVERVIVV SMNYRVGALG FLALPGNPEA PGNMGLFDQQ
210 220 230 240 250
LALQWVQKNI AAFGGNPKSV TLFGESAGAA SVSLHLLSPG SHSLFTRAIL
260 270 280 290 300
QSGSFNAPWA VTSLYEARNR TLNLAKLTGC SRENETEIIK CLRNKDPQEI
310 320 330 340 350
LLNEAFVVPY GTPLSVNFGP TVDGDFLTDM PDILLELGQF KKTQILVGVN
360 370 380 390 400
KDEGTAFLVY GAPGFSKDNN SIITRKEFQE GLKIFFPGVS EFGKESILFH
410 420 430 440 450
YTDWVDDQRP ENYREALGDV VGDYNFICPA LEFTKKFSEW GNNAFFYYFE
460 470 480 490 500
HRSSKLPWPE WMGVMHGYEI EFVFGLPLER RDNYTKAEEI LSRSIVKRWA
510 520 530 540 550
NFAKYGNPNE TQNNSTSWPV FKSTEQKYLT LNTESTRIMT KLRAQQCRFW
560 570 580 590 600
TSFFPKVLEM TGNIDEAEWE WKAGFHRWNN YMMDWKNQFN DYTSKKESCV
602
GL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3902 Collapse assay details

Data source: Panorama

Official Gene Symbol
Bche
Peptide Sequence
YLTLNTEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
529
Peptide End
536
CPTAC ID
non-CPTAC-3902
Peptide Molecular Mass
980.5179
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

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Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b4 (2+) 128.1 126.1 116.3 166.7 121.8 121.2 210.2 175.3 168 15 15 15
b2 (1+) 8.4 4.4 5.4 11.1 5 7.5 13.9 6.7 9.2 15 15 15
y3 (1+) 39.7 11.2 6.1 56.7 12.3 7.9 69.2 16.6 10 15 15 15
y6 (1+) 16 3 2.4 13.3 8.1 5.7 20.8 8.6 6.2 15 15 15
y7 (1+) 24.5 11.3 8.4 23.8 11.2 9 34.2 15.9 12.3 15 15 15
sum 9.5 2.5 1.9 13 6.3 5.1 16.1 6.8 5.4 15 15 15

Additional Resources and Comments

Resources
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Comments

Assay Details for non-CPTAC-2645 Collapse assay details

Data source: Panorama

Official Gene Symbol
BCHE
Peptide Sequence
YLTLNTESTR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
528
Peptide End
537
CPTAC ID
non-CPTAC-2645
Peptide Molecular Mass
1,196.6037
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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view larger image
view larger image

Response Curves

Data source: Panorama

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Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 10.8 6.9 4.8 13.8 11.1 7.9 17.5 13.1 9.2 15 15 15
b2 (1+) 12.1 6.2 4.9 17.1 7.7 9.4 20.9 9.9 10.6 15 15 15
y8 (1+) 14.1 6.4 5.3 13.9 8.2 7 19.8 10.4 8.8 15 15 15
y7 (1+) 10.5 5.7 8.1 16.8 9.7 8 19.8 11.3 11.4 15 15 15
b3 (1+) 9.3 7.6 8.6 11.9 12.9 9.2 15.1 15 12.6 15 15 15
sum 5.3 4.3 4.6 9.4 8.1 7.3 10.8 9.2 8.6 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments